tailieunhanh - Báo cáo y học: " Inhibitory effect of ribbon-type NF-κB decoy oligodeoxynucleotides on"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Inhibitory effect of ribbon-type NF-κB decoy oligodeoxynucleotides on . | Available online http content 8 4 R103 Research article Inhibitory effect of ribbon-type NF-kB decoy oligodeoxynucleotides on osteoclast induction and activity in vitro and in vivo Yasuo Kunugiza1 2 Tetsuya Tomita2 Naruya Tomita3 Ryuichi Morishita1 and Hideki Yoshikawa2 Division of Clinical Gene Therapy Osaka University Graduate School of Medicine 2-2 Yamada-oka Suita Osaka 565-0871 Japan 2Department of Orthopaedics Osaka University Graduate School of Medicine 2-2 Yamada-oka Suita Osaka 565-0871 Japan 3Division of Nephrology Department of Internal Medicine Kawasaki Medical School 577 Matsushima Kurashiki Okayama 701-0192 Japan Corresponding author Tetsuya Tomita tomita@ Received 11 Oct 2005 Revisions requested 1 Dec 2005 Revisions received 27 Feb 2006 Accepted 29 May 2006 Published 3 Jul 2006 Arthritis Research Therapy 2006 8 R103 doi ar1980 This article is online at http content 8 4 R103 2006 Kunugiza et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract In this study we examined the effect of ribbon-type circulartype NF-kB decoy oligodeoxynucleotides RNODN on osteoclast induction and activity. We extracted bone marrow cells from the femurs of rats and incubated non-adherent cells with receptor activator of nuclear factor kB ligand RANKL and macrophage colony-stimulating factor M-CSF . First transfer efficiency into osteoclasts and their precursors resistance to exonuclease and binding activity of decoy to NF-kB were examined. Next to examine the effect of RNODN on osteoclast induction and activity osteoclast differentiation and pit formation assays were performed. RNODN were injected into the ankle joints of rats with .