tailieunhanh - Báo cáo y học: " Functional inhibition of NF-κB signal transduction in αvβ3 integrin expressing endothelial cells by using RGD-PEG-modified adenovirus with a mutant IκB gene"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Functional inhibition of NF-κB signal transduction in αvβ3 integrin expressing endothelial cells by using RGD-PEG-modified adenovirus with a mutant IκB gene. | Available online http content 8 1 R32 Research article Functional inhibition of NF-kB signal transduction in avp3 integrin expressing endothelial cells by using RGD-PEG-modified adenovirus with a mutant IkB gene Ken-ichi Ogawara1 Joanna M Kuldo2 Koen Oosterhuis3 Bart-Jan Kroesen2 Marianne G Rots3 Christian Trautwein4 Toshikiro Kimura1 Hidde J Haisma3 and Grietje Molema2 Department of Pharmaceutics Faculty of Pharmaceutical Sciences Okayama University Okayama Japan 2University of Groningen Department of Pathology and Laboratory Medicine Medical Biology Section The Netherlands 3Department of Therapeutic Gene Modulation Groningen University Institute for Drug Exploration Groningen The Netherlands 4III Medical Clinic University Hospital of RWTH Aachen Germany Corresponding author Ken-ichi Ogawara ogawara@ Received 6 Oct 2005 Revisions requested 30 Nov 2005 Revisions received 9 Dec 2005 Accepted 14 Dec 2005 Published 13 Jan 2006 Arthritis Research Therapy 2006 8 R32 doi ar1885 This article is online at http content 8 1 R32 2006 Ogawara et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract In order to selectively block nuclear factor kB NF-kB -dependent signal transduction in angiogenic endothelial cells we constructed an avP3 integrin specific adenovirus encoding dominant negative IkB dnIKB as a therapeutic gene. By virtue of RGD modification of the PEGylated virus the specificity of the cell entry pathway of adenovirus shifted from coxsacki-adenovirus receptor dependent to avP3 integrin dependent entry. The therapeutic outcome of delivery of the transgene into endothelial cells was determined by analysis of cellular responsiveness .