tailieunhanh - Extracellular vesicles mediated proinflammatory macrophage phenotype induced by radiotherapy in cervical cancer

Radiotherapy is a highly effective treatment for cervical cancer. Recent studies focused on the radiotherapy induced anti-tumor immunity. Whether tumor-derived extracellular vesicles (EVs) play roles in radiotherapy induced tumor associated macrophage (TAM) polarization remains unclear. | Ren et al. BMC Cancer 2022 22 88 https s12885-022-09194-z RESEARCH Open Access Extracellular vesicles mediated proinflammatory macrophage phenotype induced by radiotherapy in cervical cancer Junli Ren1 Lili Li1 Baofeng Yu2 Enwei Xu3 Naiping Sun1 Xiaoning Li2 Zihan Xing2 Xiaodong Han1 Yaqin Cui1 Xiaoyan Wang4 Xiaoxue Zhang5 and Guoliang Wang6 Abstract Background Radiotherapy is a highly effective treatment for cervical cancer. Recent studies focused on the radio- therapy induced anti-tumor immunity. Whether tumor-derived extracellular vesicles EVs play roles in radiotherapy induced tumor associated macrophage TAM polarization remains unclear. Materials and Methods This study analysed the phenotype of macrophages in cancer tissue and peripheral blood of cervical cancer patients using flow cytometry analysis. The role of EVs from plasma of post-irradiated patients on M2-like transformed macrophages was assessed. The M1- and M2-like macrophages were assessed by expression of cell surface markers CCR7 CD163 and intracellular cytokines IL-10 TNFα and iNOS . The capacity of phagocytosis was assessed by PD-1 expression and phagocytosis of pHrodo Red E. coli bioparticles. Results Our results demonstrated that radiotherapy of cervical cancer induced an increase in the number of TAMs and a change in their subtype from the M2-like to the M1-like phenotype increased expression of CCR7 and decreased expression of CD163 . The EVs from plasma of post-irradiated patients facilitated the M2-like to the M1-like phenotype transition increased expression of CCR7 TNFα and iNOS and decreased expression of CD163 and IL-10 and increased capacity of phagocytosis decreased PD-1 expression and increased phagocytosis of pHrodo Red E. coli bioparticles . Conclusions Our data demonstrated that irradiation in cervical cancer patients facilitated a proinflammatory macrophage phenotype which could eventually able to mediate anti-tumor immune responses. Our findings high- light the