tailieunhanh - Isolation and characterisation of a new alkali halotolerant bacillus aquimaris strain lgmt10, producing extracellular hydrolases, from the effluents of a thermal power plant in Algeria

the results have shown that among twelve isolated bacterial strains, three strains were screened based on their enzymatic potential, and were later identified by 16S rRNA gene sequencing, . showing that the strains belong to the genus Pseudomonas aeruginosa, and Bacillus wiedmannii with a similarity percentage of and 100%, respectively, with their corresponding type of strains. | ISOLATION AND CHARACTERISATION OF A NEW ALKALI-HALOTOLERANT BACILLUS AQUIMARIS STRAIN LGMT10 PRODUCING EXTRACELLULAR HYDROLASES FROM THE EFFLUENTS OF A THERMAL POWER PLANT IN ALGERIA Abdelkrim Chaida1 Farid Bensalah1 Amel Guermouche M rassi1 Address es 1 Laboratory of Microbial Genetics LGM Department of Biology Faculty of Natural and Life Sciences University Oran 1 31000 Oran Algeria. Corresponding author https ARTICLE INFO ABSTRACT Received 16. 7. 2020 Modern biotechnology takes into account cost performance and respect for the environment to set up an industrial process. In this sense Revised 26. 6. 2021 the study s goal is to highlight the presence of indigenous microbial microflora in the Terga thermal power plant effluents which are Accepted 1. 7. 2021 capable of secreting extracellular hydrolases. Four different extracellular hydrolases classes which are most of the time used in Published bioindustry namely protease amylase lipase and cellulase were investigated in agar plate assay to select microorganisms with an interesting enzymatic potential adapted to this type of environment. Consequently the results have shown that among twelve isolated bacterial strains three strains were screened based on their enzymatic potential and were later identified by 16S rRNA gene sequencing Regular article . showing that the strains belong to the genus Pseudomonas aeruginosa and Bacillus wiedmannii with a similarity percentage of and 100 respectively with their corresponding type of strains. Among them the strain LGMT10 that belongs to the Bacillus genus and is closely related to Bacillus aquimaris showed a 16S rRNA sequence similarity with the type of strain of . This strain presents adequate characteristics to resist the harsh conditions of pH and NaCl. It could grow against wide ranges of NaCl concentrations between 0-12 w v pH and was able to produce extracellular hydrolases . .