tailieunhanh - Nghiên cứu điều kiện thu nhận Chondroitin sulfate (CS) từ sự thủy phân sụn ức gà bằng enzyme Alcalase

Bài viết xác định điều kiện thủy phân tối ưu sụn ức gà bằng enzyme Alcalase để hiệu suất thi nhận Glycosaminoglycans sulphate đạt cao nhất. | 117 2017 086-092 Nguy n Th Ng c Bình Tr nh Th Th a Ân Nguy n Uy Th ng Th i h c Bách k i h c Qu c gia Thành ph H Chí Minh n Tòa so n 20-6-2016 ch p nh -02-2017 Tóm t t Glycosaminoglycans sulphate GAGs s . là Chondroitin sulfate CS . Glycosaminoglycans sulphate sulphate .K o 4 v wpro ian 212 phút. Glycosaminoglycans sulfate 27 7 Glycosaminoglycans sulfate Glycosaminoglycans sulfate là 53 61 Chondroitin sulfate Abstract Glycosaminoglycans sulphate GAGs belonging to a family of proteoglycans are complex natural polysacchrides in animals. Chondroitin sulfate CS is a major class of Glycosaminoglycans sulphate used for treatment of arthritis especial osteoarthritis. Glycosaminoglycans sulphate can be extracted from cartilage of as cows pigs chicken sharks etc. The extraction of Glycosaminoglycans sulphate from agricultural waste sources for food supplements is meaningful for Vietnam to substitute the exported material. Our research is aimed to determine the optimal condition of hydrolysis of chicken keel cartilage by Alcalase L to obtain Glycosaminoglycans sulphate. The optimal values for the hydrolysis process are pH at 7 0 temperature at 540C the ratio of enzyme and substances at 4 v wpro and hydrolysis time of 212 minutes. Meanwhile Glycosaminoglycans sulphate sulfate content are obtained according to the predictions of the model at 27 78 dry weight of raw material . Glycosaminoglycans sulphate powder product was found to be Glycosaminoglycans sulphate 53 61 dry basis Chondroitin sulfate determined by HPLC 37 32 dry basis . Keywords Chondroitin sulfate Glycosaminoglycan Alcalase chicken keel cartilage hydrosis. 117 2017 086-092 . nh h at tính c a enzyme Alcalase o o . ng GAGs sulfate Carcharhinus Sorrah Dasyatis Kuhlii . V t li u o Bacillus Licheniformis. . Phân tích th ng kê s li u . B trí thí nghi m . Thành ph n c a s n c gà o pro . tích . nh thành ph n c a s n c gà 117 2017 086-092 . ng c a pH n ng GAGs GAGs GAGs . ng