tailieunhanh - A comparative study of microplate agglutination test (mat) with enzyme linked immunosorbant assay (ELISA) for diagnosis of brucellosis

The study was conducted in Department of Microbiology RIMS, Raichur. Blood samples were collected from the patients attending orthopedic OPD of RIMS teaching hospital with complaints of fever, joint pain and backache. RBPT, MAT and ELISA IgM and IgG were carried out on serum samples. | 2017 6 10 1550-1558 International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 6 Number 10 2017 pp. 1550-1558 Journal homepage http Original Research Article https A Comparative Study of Microplate Agglutination Test MAT with Enzyme Linked Immunosorbant Assay ELISA for Diagnosis of Brucellosis R. Venkatesh Naik1 Harish Murthy2 . Prakash1 and . Peerapur1 1 Department of Microbiology 2Department of Orthopedics RIMS Raichur Karnataka India Corresponding author ABSTRACT Brucellosis is one of the important Zoonotic diseases in India and continues to be a major Keywords public health concern globally. Human brucellosis is difficult to diagnose and requires laboratory testing for confirmation. Previously Standard Agglutination Test SAT and Brucellosis Rose Bengal Plate test Rose Bengal Plate Test RBPT were used to detect Brucellosis. Objective of the study is RBPT Microplate the determination of antibody titres in the positive sera by ELISA and MAT. The study Agglutination Test was conducted in Department of Microbiology RIMS Raichur. Blood samples were MAT and Enzyme- collected from the patients attending orthopedic OPD of RIMS teaching hospital with Linked Immunosorbant Assay complaints of fever joint pain and backache. RBPT MAT and ELISA IgM and IgG were ELISA . carried out on serum samples. It was done using Chi square test. Out of 116 blood samples tested 23 cases were positive for Brucella antibodies by RBPT and MAT. Twenty two Article Info cases were positive for ELISA IgM and twenty cases were positive for ELISA IgG. All the Accepted 40 healthy controls were negative by RBPT MAT and ELISA. Considering ELISA as 14 September 2017 gold standard sensitivity and specificity of MAT when compared to IgM ELISA was 95 Available Online and respectively. Sensitivity and specificity of MAT when compared to IgG 10 October 2017 ELISA was and .