tailieunhanh - Báo cáo khoa học: Human skin cell stress response to GSM-900 mobile phone signals In vitro study on isolated primary cells and reconstructed epidermis

In recent years, possible health hazards due to radiofrequency radiation (RFR) emitted by mobile phones have been investigated. Because several publications have suggested that RFR is stressful, we explored the potential biological effects of Global System for Mobile phone communication at 900 MHz (GSM-900) exposure on cultures of isolated human skin cells and human reconstructed epidermis (hRE) using human keratinocytes. | ỊFEBS Journal Human skin cell stress response to GSM-900 mobile phone signals In vitro study on isolated primary cells and reconstructed epidermis Sandrine Sanchez1 Alexandra Milochau2 Gilles Ruffie1 Florence Poulletier de Gannes1 Isabelle Lagroye1 3 Emmanuelle Haro1 Jean-Etienne Surleve-Bazeille2 Bernard Billaudel1 Maguy Lassegues2 and Bernard Veyret1 3 1 Bordeaux 1 University Physics of Wave-Matter Interaction PIOM Laboratory ENSCPB Pessac France 2 Bordeaux 1 University Laboratory of CellDefence and Regulation Factors EA1915 Talence France 3 Bioelectromagnetics Laboratory EPHE ENSCPB Pessac France Keywords fibroblasts keratinocytes mobile phone signal skin 3D skin model Correspondence S. Sanchez Physics of Wave-Matter Interaction PIOM Laboratory ENSCPB 16 Avenue Pey-Berland F-33607 Pessac Cedex France Fax 33 5 40 00 66 31 Tel 33 5 40 00 69 65 E-mail Received 31 July 2006 revised 10 October 2006 accepted 17 October 2006 doi In recent years possible health hazards due to radiofrequency radiation RFR emitted by mobile phones have been investigated. Because several publications have suggested that RFR is stressful we explored the potential biological effects of Global System for Mobile phone communication at 900 MHz GSM-900 exposure on cultures of isolated human skin cells and human reconstructed epidermis hRE using human keratinocytes. As cell stress markers we studied Hsc70 Hsp27 and Hsp70 heat shock protein HSP expression and epidermis thickness as well as cell proliferation and apoptosis. Cells were exposed to GSM-900 under optimal culture conditions for 48 h using a specific absorption rate SAR of 2 W kg-1. This SAR level represents the recommended limit for local exposure to a mobile phone. The various biological parameters were analysed immediately after exposure. Apoptosis was not induced in isolated cells and there was no alteration in hRE thickness or proliferation. No change in HSP expression was .