tailieunhanh - Báo cáo y học: " Peroxisome Proliferator-Activated Receptor α (PPARα) down-regulation in cystic fibrosis lymphocytes"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Peroxisome Proliferator-Activated Receptor α (PPARα) down-regulation in cystic fibrosis lymphocytes. | Respiratory Research BioMed Central Open Access Peroxisome Proliferator-Activated Receptor a PPARa down-regulation in cystic fibrosis lymphocytes Veerle Reynders 1 Stefan Loitsch1 Constanze Steinhauer1 Thomas Wagner1 Dieter Steinhilber2 and Joachim Bargon1 Address 1Dept. of Internal Medicine Division of Pneumology University Hospital Frankfurt Germany and institute of Pharmaceutical Chemistry University of Frankfurt Frankfurt am Main Germany Email Veerle Reynders - veerlereynders@ Stefan Loitsch - sm_loitsch@ Constanze Steinhauer - consti24@ Thomas Wagner - Dieter Steinhilber - steinhilber@ Joachim Bargon - bargon@ Corresponding author Published 30 July 2006 Received 16 February 2006 Respiratory Research 2006 7 104 doi 1465-9921-7-104 Accepted 30 July 2006 This article is available from http content 7 1 104 2006 Reynders et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract_ Background PPARs exhibit anti-inflammatory capacities and are potential modulators of the inflammatory response. We hypothesized that their expression and or function may be altered in cystic fibrosis CF a disorder characterized by an excessive host inflammatory response. Methods PPARa p and Y mRNA levels were measured in peripheral blood cells of CF patients and healthy subjects via RT-PCR. PPARa protein expression and subcellular localization was determined via western blot and immunofluorescence respectively. The activity of PPARa was analyzed by gel shift assay. Results In lymphocytes the expression of PPARa mRNA but not of PPARp was .