tailieunhanh - Genome mining reveals chitin degradation potential of streptomyces parvulus VCCM 22513

In the present study, out of 22 Streptomyces strains, Streptomyces parvulus VCCM 22513 produced the highest chitinase activity. Time courses of incubation revealed that the maximum chitinase ( ± U/mL) of this strain was observed after 96 hours in the yeast extract salts medium supplemented with g/L colloidal chitin. | ACADEMIA JOURNAL OF BIOLOGY 2023 45 2 27 36 DOI 2615-9023 18027 GENOME MINING REVEALS CHITIN DEGRADATION POTENTIAL OF Streptomyces parvulus VCCM 22513 Quach Ngoc Tung1 2 Nguyen Thi Thu An1 Vu Thi Hanh Nguyen1 2 Phi Quyet Tien1 2 1 Institute of Biotechnology VAST Vietnam 2 Graduate University of Science and Technology VAST Vietnam Received 13 January 2023 accepted 24 May 2023 ABSTRACT The genus Streptomyces is not only known as a natural producer of antibiotics but also a prolific source of chitinolytic enzymes that digest recalcitrant chitin to chitooligosaccharides. However only a few reports have used whole-genome sequencing to study chitin degradation of Streptomyces to date. In the present study out of 22 Streptomyces strains Streptomyces parvulus VCCM 22513 produced the highest chitinase activity. Time courses of incubation revealed that the maximum chitinase U mL of this strain was observed after 96 hours in the yeast extract salts medium supplemented with g L colloidal chitin. Additional genomic analysis of VCCM 22513 was also conducted to discover the genomic information related to chitin degradation. The VCCM 22513 genome consists of 341 carbohydrate-active enzyme coding genes divided into 6 families including glycoside hydrolase 134 genes carbohydrate-binding module 88 genes glycosyl transferase 87 genes carbohydrate esterase 18 genes polysaccharide lyase 7 genes and auxiliary activity 7 genes . Further genome mining revealed the presence of 10 chitinases 4 lytic polysaccharide monooxygenases and 14 β-N- acetylhexosaminidases which mainly contribute to the degradation of chitin polymers. This is the first report revealing the mechanism underlying the chitin degradation of S. parvulus. Future investigations are required to characterize chitinolytic enzymes found in this study for the bioeconomic production of high-quality chitooligosaccharides from chitin food wastes. Keywords CAZy chitin degradation chitinases .