tailieunhanh - Chọn lọc sau biến nạp gen AtZAT12 trên cây Arabidopsis

Nghiên cứu này nhằm chọn lọc các hạt Arabidopsis sau chuyển gen AtZAT12 gián tiếp qua Agrobacterium tumerfaciens. ZAT12 là một yếu tố phiên mã có chức năng ức chế yếu tố phiên mã khác là FIT thông qua motif EAR. | 32 Trường Đại học Nông Lâm TP. Hồ Chí Minh Selection of Arabidopsis transformants containing AtZAT12 Cham T. T. Le amp Ngoc T. Pham Faculty of Agronomy Vietnam National University of Agriculture Ha Noi Vietnam ARTICLE INFO ABSTRACT Research Paper This study aimed to select Arabidopsis seeds after transformation with the AtZAT12 gene via Agrobacterium tumerfaciens. ZAT12 is a transcription Received February 19 2023 factor that inhibits other transcription factors FIT through the EAR Revised July 11 2023 motif. FIT itself is a central transcription factor that controls Fe uptake Accepted July 13 2023 under Fe-deficient conditions. However if Fe is absorbed excessively it will produce reactive oxygen species that could damage cells. That is the Keywords reason why AtZAT12 transcription is elevated and FIT transcription is inhibited under Fe deficiency for 10 days. To investigate the function the AtZAT12 gene AtZAT12 gene was inserted into the pMDC107 vector for transformation Hygromycin B into Arabidopsis. The T0 Arabidopsis seeds obtained after floral dip Long hypocotyls transformation were placed on 1 MS agar supplemented with pMDC107 15 μg mL Hygromycin B. Beforehand the T0 seeds were sterilized and Transformed Arabidopsis kept in the dark for stratification. Subsequently the seed plates were subjected to a regime of 6 h of light 48 h of dark and 24 h of light d . Corresponding author The hygromycin B-resistant seedlings had long hypocotyls cm Le Thi Tuyet Cham while the non-resistant seedlings had short cm hypocotyls. This Email lttcham@ method took only days to identify transgenic Arabidopsis seedlings. After that positive transgene lines were examined by PCR method for AtZAT12 and the expression of AtZAT12 was observed under microscope. Cited as Le C. T. T. amp Pham N. T. 2023 . Selection of Arabidopsis transformants containing AtZAT12. The Journal of Agriculture and Development 22 4 32-39. Tạp chí Nông nghiệp và Phát triển 22 4 .