tailieunhanh - Phân biệt biến thể lan Kiều tím (Dendrobium amabile) bằng đặc điểm hình thái và chỉ thị ITS

Bài viết Phân biệt biến thể lan Kiều tím (Dendrobium amabile) bằng đặc điểm hình thái và chỉ thị ITS trình bày kết quả phân tích hình thái thực vật của 4 biến thể lan Kiều tím của Việt Nam. Đồng thời, bài báo cũng xác định và phân tích trình tự DNA barcode ITS của 2 biến thể đại diện Kiều tím miền trung và Kiều tím bắc. | TNU Journal of Science and Technology 228 05 423 - 430 DISTINGUISHING OF Dendrobium amabile STRAIN BY MORPHOLOGYCAL AND ITS MARKER Nguyen Thi Hai Yen1 Ngo Xuan Quang2 Nguyen Thi Loan3 Chu Hoang Mau4 1TNU University of Sciences 2Institute of Tropical Biology - VAST 3Hoa Lu University 4TNU University of Education ARTICLE INFO ABSTRACT Received 09 3 2023 Kieu orchid is a group of genus Dendrobium orchids belonging to the tribe Chrysotoxae section Callista. Currently the classification of the Kieu orchid Revised 19 4 2023 group is still not unified. There are different views about the number of Kieu Published 28 4 2023 orchid species in Vietnam as well as their kinship. This species Dendrobium amabile is divided into 2 groups with large different conservation and economic values which are namely the Central Purple Kieu and the Northern Purple Kieu. KEYWORDS Our paper focus to investigate and find out results of morphological analysis of 4 Distingsh varieties of purple Kieu orchid of Vietnam. Furthermore identifying and analyzing the ITS sequences of the two variants Kieu violet in the central region Strain and Kieu violet in Thai Nguyen Province. Morphological analysis also showed Dendrobium amabile that the northern varieties of the Kieu violet differ from the characteristics of the ITS Kieu violet described in the botanical documents in some morphological details and distribution. Moreover results of DNA analysis of ITS markers showed that Kieu orchid the Central Purple Kieu has a sequence similarity of comparing to the ITS sequence of species Dendrobium amabile published on the GenBank while the Northern Purple Kieu variant has lower similarity and much different in the ITS sequence. However tree they all belong to a clade with the species Dendrobium amabile and are separated from the rest in the taxonomic. Hence our results indicate that these taxa could be new species to have further studies to determine the scientific name for the species variable for the .