tailieunhanh - Phân tích chỉ thị DNA ITS và matK trên một số loài Đinh lăng của Việt Nam

Trong nghiên cứu này, hai vùng gen ITS và matK được khuếch đại, giải trình tự và phân tích nhằm định danh và tìm hiểu các vùng DNA chỉ thị. Nghiên cứu tiến hành với 5 mẫu đinh lăng, gồm Đinh lăng lá nhỏ (P. fruticosa), Đinh lăng lá đĩa P. scutellaria, Đinh lăng lá răng P. serrata Balf, Đinh lăng lá to P. filicifolia, Đinh lăng lá tròn P. balfouriana. | TNU Journal of Science and Technology 227 05 318 - 325 ANALYSIS OF DNA BARCODE REGION ITS AND MATK IN SOME POLYSCIAS PLANTS OF VIETNAM Huynh Thi Thu Hue1 2 Nguyen Thi Bich Ngoc1 Dao Quang Ha1 Le Thi Thu Hien1 2 Nguyen Xuan Canh3 1Institute of Genome Research VAST 2Graduate University of Science and Technology - VAST 3Vietnam National University of Agriculture ARTICLE INFO ABSTRACT Received 01 3 2022 Many polyscias species are herbal plants belonging to the Polycias genus which are valuable in traditional medicine. Active ingredients in leaves and Revised 29 4 2022 roots of Policias plant includes saponins vitamins and amino acid that are Published 29 4 2022 essential compounds in herb medicine. Although these species have been studied and used for many years studies on genetic diversity are limited. KEYWORDS Nowadays with the development of barcoding DNA technology the genetic diversity of plants has begun to be studied at the molecular level instead of Polycias based on morphological characteristics. The study was conducted with five DNA barcoding samples including P. fruticosa P. scutellaria P. serrata Balf P. filicifolia P. ITS balfouriana. In this research we performed amplification sequencing and analysis of ITS and MatK regions to identify the marker DNA regions for MatK these species. The results showed the ITS and MatK sequences of these five Sequencing species had high similarities with polyscias sequences on the Gene Bank. In addition the ITS region has many DNA polymorphisms in sequence. The distinguished by ITS sequence is more clear than the MatK sequence in terms of genetic distance between species of the Polyscias genus and the Araliaceae family. These results provide the DNA barcoding sequences to contribute to the research on evaluation taxonomy and gene conservation in medicinal plants of the Policias genus. PHÂN TÍCH CHỈ THỊ DNA ITS VÀ MATK TRÊN MỘT SỐ LOÀI ĐINH LĂNG CỦA VIỆT NAM Huỳnh Thị Thu Huệ1 2 Nguyễn Thị Bích Ngọc1 Đào Quang Hà1 Lê Thị .