tailieunhanh - Khảo sát hiệu quả bảo vệ bức xạ cho tế bào lympho người và nguyên bào sợi người của liposome đóng gói resveratrol

Nghiên cứu nhằm mục đích đánh giá hiệu quả bảo vệ bức xạ của liposome đóng gói resveratrol (RES-Lip). Đầu tiên, Lip-RES được tổng hợp bằng phương pháp hydrate hóa màng lipid kết hợp với sóng siêu âm để giảm kích thước. | TNU Journal of Science and Technology 227 05 137 - 145 STUDY ON RADIOPROTECTION EFFECT FOR HUMAN LYMPHOCYTES AND FIBROBLASTS OF RESVERATROL-ENCAPSULATED LIPOSOME Nguyen Minh Hiep1 Pham Ngoc Duy1 Vu Ngoc Bich Dao1 Tran Thanh Mai1 Tran Thi Ngoc Mai1 Nguyen Thi Huynh Nga2 1Center of Radiation Technology and Biotechnology 2Dalat University ARTICLE INFO ABSTRACT Received 19 02 2022 This study aims to evaluate radioprotection effect of resveratrol- encapsulated liposomes Lip-RES . Firstly Lip-RES was prepared by a Revised 18 4 2022 combining method of lipid-film hydration method and sonication. Published 18 4 2022 Penetration of Lip-RES into human lymphocytes and human fibroblasts HF cells was observed by fluorescene microscopy. The results KEYWORDS demonstrated that Lip-RES was successfully prepared with a relatively spherical shape good characteristics mean particle size of nm Fibroblasts polydispersity index of zeta potential of mV encapsulation Liposome efficiency of and payload of and a relatively high stability. Lip-RES was well penetrated into human lymphocytes and HF Lymphocytes cells. Under non-irradiated condition Lip-RES showed a negligible Radioprotection genotoxicity but exhibited a significant cytotoxicity for lymphocytes and Resveratrol HF cells at RES concentration of more than 10 µg mL. Under irradiated condition at a dose of 2 Gy compared to the control Lip-RES could decrease the percentage of cell death of 16-30 and decrease the number of foci cell of 10-15 . This indicates the radioprotection effect of Lip- RES for human lymphocytes and fibroblasts. Moreover at a RES concentration of µg mL Lip-RES showed a best radioprotection. KHẢO SÁT HIỆU QUẢ BẢO VỆ BỨC XẠ CHO TẾ BÀO LYMPHO NGƯỜI VÀ NGUYÊN BÀO SỢI NGƯỜI CỦA LIPOSOME ĐÓNG GÓI RESVERATROL Nguyễn Minh Hiệp1 Phạm Ngọc Duy1 Vũ Ngọc Bích Đào1 Trần Thanh Mai1 Trần Thị Ngọc Mai1 Nguyễn Thị Huỳnh Nga2 1Viện Nghiên cứu Hạt nhân 2Trường Đại học Đà Lạt THÔNG TIN BÀI BÁO TÓM .