tailieunhanh - The DNA barcoding data and genetic distance of leaf beetles (Coleoptera, Chrysomelidae) in Vietnam

DNA barcoding is a useful tool in identifying species, biodiversity assessment, and revealing phylogenetic relationships of living organisms in the world. However, the DNA barcode data for leaf beetles in Vietnam is lacking. In this study, sixteen DNA sequences of 658 bp of COI gene from nine species (five genera; three subfamilies) of Chrysomelidae in Vietnam were (obtained). Intra- and inter-specific diversities, and phylogenetic relationships of these species were analyzed. | ACADEMIA JOURNAL OF BIOLOGY 2020 42 4 25 31 DOI 2615-9023 THE DNA BARCODING DATA AND GENETIC DISTANCE OF LEAF BEETLES Coleoptera Chrysomelidae IN VIETNAM Nguyen Thi Dinh Institute of Ecology and Biological Resources VAST Vietnam Received 8 June 2020 accepted 20 September 2020 ABSTRACT DNA barcoding is a useful tool in identifying species biodiversity assessment and revealing phylogenetic relationships of living organisms in the world. However the DNA barcode data for leaf beetles in Vietnam is lacking. In this study sixteen DNA sequences of 658 bp of COI gene from nine species five genera three subfamilies of Chrysomelidae in Vietnam were obtained . Intra- and inter-specific diversities and phylogenetic relationships of these species were analyzed. Keywords Biodiversity COI gene intra- and inter-species diversity molecular identification tropical forest. Citation Nguyen Thi Dinh 2020. The dna barcoding data and genetic distance of leaf beetles Coleoptera Chrysomelidae in Vietnam. Academia Journal of Biology 42 4 25 31. https 2615- 9023 Corresponding author email 2020 Vietnam Academy of Science and Technology VAST 25 Nguyen Thi Dinh INTRODUCTION 2016 . Among these species only 18 species The family Chrysomelidae is one of the were identified to species level by morphology largest family of the order Coleoptera with Nguyen amp Gómez-Zurita 2017 remaining 35 000 60 000 species in the world Schmitt 123 morphological form were only identified 1996 Futuyma 2004 Splipnski et al. 2011 to subfamily or genus level. Jolivet 2015 . This study generated and submitted sixteen DNA barcoding was proposed as a method DNA sequences of 658 bp of COI of nine of species identification Hebert et al. 2003 . It species belonging to five genera of three uses a short standard fragment 658 bp of subfamilies that were collected in several mitochondrial cytochrome oxidase subunit I provinces in Vietnam. These are the .

• Sinh học
• COI gene
• Intra and inter species diversity
• Molecular identification
• Tropical forest
• Genetic distance of leaf beetles
• Cá rô đồng
• Cây phát sinh loài
• Đa dạng di truyền
• Gene COI
• Cá thuộc chi Anabas
• Agriculture and rural development
• Morphological characteristics of the green water dragon
• Mitochondrial COI gene of the green water dragon
• The green water dragon
• Physignathus cocincinus
• BMC Genomics
• Triticum aestivum L
• Gene family
• JA signaling
• Male sterile
• Quantitative real time PCR
• Journal of Zoology
• Codling moth
• Genetic diversity
• Phylogenetic analyses
• Mã vạch DNA
• Loài tu hài
• Cytochrome c oxidase subunit I
• Lutraria rhychaena
• Trình tự vùng gene COI