tailieunhanh - Somatic embryogenesis from mature seed cultures of Pistacia atlantica

Embryogenic mass was produced from kernels of mature fruits of Pistacia atlantica cultured in liquid Murashige and Skoog media, supplemented with 100 mg/l casein hydrolysate, 100 mg/l l-ascorbic acid, and benzylaminopurine (BAP). Embryogenic masses were differentiated directly from the kernel explants after culture for 3 weeks in liquid medium with mg/l benzylaminopurine. | Turk J Agric For 24 (2000) 465–473 © TÜBİTAK Somatic Embryogenesis From Mature Seed Cultures of Pistacia atlantica Ahmet ONAY Dicle University, Faculty of Science and Art, Department of Biology, Diyarbakır-TURKEY Received: Abstract: Embryogenic mass was produced from kernels of mature fruits of Pistacia atlantica cultured in liquid Murashige and Skoog media, supplemented with 100 mg/l casein hydrolysate, 100 mg/l l-ascorbic acid, and benzylaminopurine (BAP). Embryogenic masses were differentiated directly from the kernel explants after culture for 3 weeks in liquid medium with mg/l benzylaminopurine. After transfer of the embryogenic masses into the same medium, but after a few subcultures with benzylaminopurine, somatic embryos appeared. Clusters of somatic embryos were transferred to the agar solidified MS medium for maturation. Matured somatic embryos, germinated on the maturation medium without growth regulators, developed into plantlets. Abbreviations: 2,4-D - 2,4-dichlorophenoxyacetic acid; BAP - benzylaminopurine (N6 -benzyladenine); EMS -embryogenic mass; IAA - Indole-3-acetic asid; MS - Murashige and Skoog medium; NAA - α- naphthalene acetic acid; PGR - plant growth regulator; SE(s) somatic embryo (s); Pistacia atlantica’nın Olgun Tohumlarında Somatik Embriyogenesis Özet: 100 mg/I hidrolize-kazein, 100 mg/I I-askorbik asit ve benzilaminopurin ile desteklenen sıvı Murashige ve Skoog (MS) besi ortamında kültürelınan Pistacia atlantica’nın tohumlarından embriyogenik doku rejenere edilmiştir. Embriyogenik dokular sıvı besi ortamında benzilaminopurin ( mg/I) ile kültüre alındıktan 3 hafta sonra tohum eksplantlarından direkt olarak farklılaşmışlardır. Rejenere olan dokular benzilaminopurin ile aynı besi ortamında birkaç kez altkültürü yapıldıktan sonra somatik embriyolar üretilmiştir. Olgunlaşmamış somatik embriyo grupları olgunlaşma için agarla katılaştırılmış MS besi ortamına transfer edilmiştir. Olgunlaşmış somatik embriyolar bitki

• Nông nghiệp
• Dichlorophenoxyacetic acid
• Embryogenic mass
• Murashige and Skoog medium
• Naphthalene acetic acid
• Plant growth regulator
• Somatic embryo
• Zygotic embryo
• Grape rootstock
• Vitis vinifera L
• Regenerated somatic plantlets
• Rapid sex determination
• Preimplantation bovine embryo
• Direct PCR from a single blastomere
• Sex known somatic nuclear transfer technology
• The preimplantation embryo
• Y chromosome specific
• Somatic embryogenesis
• Embryogenic calli
• Non embryogenic calli
• Cell suspension cultures
• Scanning electron microscope
• BMC Plant Biology
• Cacao zygotic embryo development
• Cacao somatic embryogenesis
• Embryogenic potential
• Fatty acid biosynthesis
• Bovine leukocyte antigene DRB3
• Cloned bovine embryo
• Molecular sexing
• Preimplantation genetic diagnosis
• Sequence based typing
• Plant tissue culture
• Wheat genetic
• Plant regeneration
• Established in soil
• Red clover
• Transmission electron microscope
• Kinetin trials
• Aseptic seedlings
• Theobroma cacao
• Zygotic embryogenesis
• Gene expression
• Báo cáo lâm nghiệp hay
• cách trình bày báo cáo
• báo cáo lâm nghiệp
• công trình nghiên cứu lâm nghiệp
• tài liệu về lâm nghiệp
• Norway spruce
• Embryonal mass
• Mature embryos
• BMC Genomics
• Transcriptional defect
• Reprogramming barrier
• SCNT embryo
• RNA seq
• Single cell RNA seq
• Somatic cell nuclear transfer
• Cell reprogramming
• Mouse embryo