tailieunhanh - Somatic embryogenesis from mature seed cultures of Pistacia atlantica

Embryogenic mass was produced from kernels of mature fruits of Pistacia atlantica cultured in liquid Murashige and Skoog media, supplemented with 100 mg/l casein hydrolysate, 100 mg/l l-ascorbic acid, and benzylaminopurine (BAP). Embryogenic masses were differentiated directly from the kernel explants after culture for 3 weeks in liquid medium with mg/l benzylaminopurine. | Turk J Agric For 24 (2000) 465–473 © TÜBİTAK Somatic Embryogenesis From Mature Seed Cultures of Pistacia atlantica Ahmet ONAY Dicle University, Faculty of Science and Art, Department of Biology, Diyarbakır-TURKEY Received: Abstract: Embryogenic mass was produced from kernels of mature fruits of Pistacia atlantica cultured in liquid Murashige and Skoog media, supplemented with 100 mg/l casein hydrolysate, 100 mg/l l-ascorbic acid, and benzylaminopurine (BAP). Embryogenic masses were differentiated directly from the kernel explants after culture for 3 weeks in liquid medium with mg/l benzylaminopurine. After transfer of the embryogenic masses into the same medium, but after a few subcultures with benzylaminopurine, somatic embryos appeared. Clusters of somatic embryos were transferred to the agar solidified MS medium for maturation. Matured somatic embryos, germinated on the maturation medium without growth regulators, developed into plantlets. Abbreviations: 2,4-D - 2,4-dichlorophenoxyacetic acid; BAP - benzylaminopurine (N6 -benzyladenine); EMS -embryogenic mass; IAA - Indole-3-acetic asid; MS - Murashige and Skoog medium; NAA - α- naphthalene acetic acid; PGR - plant growth regulator; SE(s) somatic embryo (s); Pistacia atlantica’nın Olgun Tohumlarında Somatik Embriyogenesis Özet: 100 mg/I hidrolize-kazein, 100 mg/I I-askorbik asit ve benzilaminopurin ile desteklenen sıvı Murashige ve Skoog (MS) besi ortamında kültürelınan Pistacia atlantica’nın tohumlarından embriyogenik doku rejenere edilmiştir. Embriyogenik dokular sıvı besi ortamında benzilaminopurin ( mg/I) ile kültüre alındıktan 3 hafta sonra tohum eksplantlarından direkt olarak farklılaşmışlardır. Rejenere olan dokular benzilaminopurin ile aynı besi ortamında birkaç kez altkültürü yapıldıktan sonra somatik embriyolar üretilmiştir. Olgunlaşmamış somatik embriyo grupları olgunlaşma için agarla katılaştırılmış MS besi ortamına transfer edilmiştir. Olgunlaşmış somatik embriyolar bitki