tailieunhanh - báo cáo khoa học: " Comparative analysis of expressed sequence tags (ESTs) between drought-tolerant and -susceptible genotypes of chickpea under terminal drought stress"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Comparative analysis of expressed sequence tags (ESTs) between drought-tolerant and -susceptible genotypes of chickpea under terminal drought stress | Deokar et al. BMC Plant Biology 2011 11 70 http 1471-2229 11 70 BMC Plant Biology RESEARCH ARTICLE Open Access Comparative analysis of expressed sequence tags ESTs between drought-tolerant and -susceptible genotypes of chickpea under terminal drought stress 1 2 3 3 Amit A Deokar 1 Vishwajith Kondawar1 Pradeep K Jain S Mohan Karuppayil N L Raju Vincent Vadez Rajeev K Varshney3 4 and R Srinivasan1 Abstract Background Chickpea Cicer arietinum L. is an important grain-legume crop that is mainly grown in rainfed areas where terminal drought is a major constraint to its productivity. We generated expressed sequence tags ESTs by suppression subtraction hybridization SSH to identify differentially expressed genes in drought-tolerant and -susceptible genotypes in chickpea. Results EST libraries were generated by SSH from root and shoot tissues of IC4958 drought tolerant and ICC 1882 drought resistant exposed to terminal drought conditions by the dry down method. SSH libraries were also constructed by using 2 sets of bulks prepared from the RNA of root tissues from selected recombinant inbred lines RILs 10 each for the extreme high and low root biomass phenotype. A total of 3062 unigenes 638 contigs and 2424 singletons of which were novel in chickpea were derived by cluster assembly and sequence alignment of 5949 ESTs. Only 2185 71 unigenes showed significant BLASTX similarity 1E-06 in the NCBI non-redundant nr database. Gene ontology functional classification terms BLASTX results and GO term were retrieved for 2006 sequences and 656 sequences were further annotated with 812 Enzyme Commission EC codes and were mapped to 108 different KEGG pathways. In addition expression status of 830 unigenes in response to terminal drought stress was evaluated using macro-array dot blots . The expression of few selected genes was validated by northern blotting and quantitative real-time PCR assay. Conclusion Our study compares not only genes that are

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