tailieunhanh - Báo cáo khoa học: Molecular and cellular specificity of post-translational aminoacyl isomerization in the crustacean hyperglycaemic hormone family

d-aminoacyl residues have been detected in various animal peptides from several taxa, especially vertebrates and arthropods. This unusual polymor-phism was shown to occur in isoforms of the crustacean hyperglycaemic hormone (CHH) of the American lobster because ad-phenylalanyl residue was found in position 3 of the sequence (CHH andd-Phe3 CHH). | Molecular and cellular specificity of post-translational aminoacyl isomerization in the crustacean hyperglycaemic hormone family Celine Ollivaux1 2 3 Dominique Gallois4 Mohamed Amiche4 Maryse Boscameric4 and Daniel Soyez4 1 Université Pierre et Marie Curie - Paris 06 UMR 7150 Mer et Sante Équipe Physiologie Compares des Erythrocytes Station Biologique de Roscoff France 2 Centre Nationalde la Recherche Scientifique UMR 7150 Station Biologique de Roscoff France 3 Universite Europeenne de Bretagne UEB Rennes France 4 Equipe Biogenese des Signaux Peptidiques ÉR3 Universite Paris France Keywords aminoacylisomerization confocallaser scanning microscopy crustacean hyperglycaemic hormone MALDI-TOF MS vitellogenesis inhibiting hormone Correspondence C. Ollivaux Universite Pierre et Marie Curie - Paris 06 UMR 7150 Mer et Sante Equipe Physiologie Comparee des Erythrocytes Station Biologique de Roscoff 29682 Roscoff Cedex France Fax 33 1 44 27 23 61 Tel. 33 1 44 27 22 62 E-mail Received 29 March 2009 revised 23 June 2009 accepted 26 June 2009 D-aminoacyl residues have been detected in various animal peptides from several taxa especially vertebrates and arthropods. This unusual polymorphism was shown to occur in isoforms of the crustacean hyperglycaemic hormone CHH of the American lobster because a D-phenylalanyl residue was found in position 3 of the sequence CHH and D-Phe3 CHH . In the present study we report the detailed strategy used to characterize in the lobster neuroendocrine system isomers of another member of the CHH family vitellogenesis inhibiting hormone VIH . We have demonstrated that the fourth residue is either an L- or a D- tryptophanyl residue VIH and D-Trp4 VIH . Furthermore use of antisera specifically recognizing the epimers of CHH and VIH reveals that aminoacyl isomerization occurs in specialized cells of the X organ-sinus gland neurosecretory system and that the D-forms of the two neuropeptides are not only present in the same cells