tailieunhanh - Báo cáo khoa học: Transcriptional upregulation of inflammatory cytokines in human intestinal epithelial cells following Vibrio cholerae infection

Coordinated expression and upregulation of interleukin-1a, interleukin-1b, tumor necrosis factor-a, interleukin-6, granulocyte–macrophage colony-stimulating factor, interleukin-8, monocyte chemotactic protein-1 (MCP-1) and epithelial cell derived neutrophil activator-78, with chemoattractant and proinflammatory properties of various cytokine families, were obtained in the intestinal epithelial cell line Int407 upon Vibrio choleraeinfection. | ỊFEBS Journal Transcriptional upregulation of inflammatory cytokines in human intestinal epithelial cells following Vibrio cholerae infection Arunava Bandyopadhaya Madhubanti Sarkar and Keya Chaudhuri Molecular Human Genetics Division Indian Institute of ChemicalBiology Kolkata India Keywords cholera toxin cytokines intestinalepithelial cells nuclear factor-KB Vibrio choleras Correspondence K. Chaudhuri Molecular Human Genetics Division Indian Institute of Chemical Biology Kolkata-700 032 India Fax 91 33 2473 5197 Tel 91 33 2473 3491 E-mail keyachaudhuri@ or kchaudhuri@ These authors contributed equally to this work Received 21 February 2007 revised 31 May 2007 accepted 13 July 2007 doi Coordinated expression and upregulation of interleukin-1 a interleukin-1 b tumor necrosis factor-a interleukin-6 granulocyte-macrophage colonystimulating factor interleukin-8 monocyte chemotactic protein-1 MCP-1 and epithelial cell derived neutrophil activator-78 with chemoattractant and proinflammatory properties of various cytokine families were obtained in the intestinal epithelial cell line Int407 upon Vibrio cholerae infection. These proinflammatory cytokines also showed increased expression in T84 cells except for interleukin-6 whereas a striking dissimilarity in cytokine expression was observed in Caco-2 cells. Gene expression studies of MCP-1 granulocyte-macrophage colony-stimulating factor interleukin-1a interleukin-6 and the anti-inflammatory cytokine transforming growth factor-b in Int407 cells with V. cholerae culture supernatant cholera toxin lipopolysaccharide and ctxA mutant demonstrated that apart from cholera toxin and lipopolysaccharide V. cholerae culture supernatant harbors strong inducer s of interleukin-6 and MCP-1 and moderate inducer s of inter-leukin-1a and granulocyte-macrophage colony-stimulating factor. Cholera toxin- or lipopolysaccharide-induced cytokine expression is facilitated by activation of .