tailieunhanh - Genetic structure and diversity of Adonis L. (Ranunculaceae) populations collected from Turkey by inter-primer binding site (iPBS) retrotransposon markers

The genus Adonis L. is a member of Ranunculaceae and consists of perennial and annual herbaceous plants included in the tribe Adonideae under the subfamily Ranunculoideae. Botanically, Ranunculaceae comprises vital medicinal plants. Molecular markers are one of the most effective tools for exploring genetic variation that can enhance breeding efficiency. To identify the genetic diversity of 62 Adonis ecotypes collected from different regions in Turkey, the interprimer binding site (iPBS) retrotransposon system was used. Of the 83 iPBS primers used, 10 provided sufficient polymorphic data, generating a total of 204 alleles. The number of iPBS bands per individual was , and the number of alleles per polymorphic locus ranged from 8 to 35, with an average of . | Turkish Journal of Botany Turk J Bot 2019 43 585-596 http botany TÜBİTAK Research Article doi bot-1810-1 Genetic structure and diversity of Adonis L. Ranunculaceae populations collected from Turkey by inter-primer binding site iPBS retrotransposon markers 1 2 3 4 3 1 Arash HOSSEIN-POUR Faruk KARAHAN Emre İLHAN Ahmet İLÇİM Kamil HALİLOĞLU 1 Department of Field Crops Faculty of Agriculture Atatürk University 25240 Erzurum Turkey 2 Crop and Horticultural Science Research Department Ardabil Agricultural and Natural Resources Research and Education Center AREEO Ardabil Moghan Iran 3 Department of Biology Faculty of Science Mustafa Kemal University 31100 Hatay Turkey 4 Department of Molecular Biology and Genetics Faculty of Science Erzurum Technical University 25050 Erzurum Turkey Received Accepted Published Online Final Version Abstract The genus Adonis L. is a member of Ranunculaceae and consists of perennial and annual herbaceous plants included in the tribe Adonideae under the subfamily Ranunculoideae. Botanically Ranunculaceae comprises vital medicinal plants. Molecular markers are one of the most effective tools for exploring genetic variation that can enhance breeding efficiency. To identify the genetic diversity of 62 Adonis ecotypes collected from different regions in Turkey the interprimer binding site iPBS retrotransposon system was used. Of the 83 iPBS primers used 10 provided sufficient polymorphic data generating a total of 204 alleles. The number of iPBS bands per individual was and the number of alleles per polymorphic locus ranged from 8 to 35 with an average of . The average polymorphism percentage was and polymorphic information content ranged from to . The highest average number of alleles Nei s genetic diversity h and Shannon s information index I were obtained from A. volgensis species and respectively whereas the lowest values and .