tailieunhanh - Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS

: The study aims to determine the secondary metabolites of Hypericum androsaemum L. extracts by liquid chromatographyhigh resolution mass spectrometry (LC-HRMS), and investigate the antioxidant and cytotoxic activities of the plant. Cytotoxic activity was evaluated by MTT assay, and apoptosis induction abilities on human prostate adenocarcinoma (PC-3), and hepatocellular carcinoma (Hep G2) cell lines. Accordingly, major secondary metabolites were found as hederagenin (762 ± μg/g) in the leaves dichloromethane (LD), herniarin (167 ± μg/g) in fruit dichloromethane (FD), (-)-epicatechin (6538 ± μg/g) in the leaves methanol (LM), (-)-epigallocatechin gallate (758 ± μg/g) in the fruit methanol (FM), and caffeic acid (370 ± μg/g) in the fruit water (FW), and (3313 ± μg/g) in the leaves water (LW) extracts. |

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