tailieunhanh - Optimization of culture conditions for squalene production and squalene extraction method of Thraustochytrium sp. TN22

In present study, the optimal conditions for high squalene production of Thraustochytrium sp. TN22 were investigated. Additionally, the methods for extraction and purification of squalene from cell suspension of this strain were developed. | ACADEMIA JOURNAL OF BIOLOGY 2020 42 4 73 85 DOI 2615-9023 OPTIMIZATION OF CULTURE CONDITIONS FOR SQUALENE PRODUCTION AND SQUALENE EXTRACTION METHOD OF Thraustochytrium sp. TN22 Hoang Thi Minh Hien1 Le Thi Thom1 2 Nguyen Cam Ha1 2 Hoang Thi Lan Anh1 Dang Diem Hong1 2 3 1 Institute of Biotechnology VAST 2 Graduate University of Science and Technology VAST 3 Thuyloi University Ha Noi Vietnam Received 9 April 2020 accepted 20 September 2020 ABSTRACT Squalene a natural triterpene with important roles as antioxidant skin hydrating cardio - protection and detoxifier has attracted the attention of researchers in the world. This study investigated the optimal culture conditions for squalene production and to develop a method for squalene extraction and purification from cell suspension of Thraustochytrium sp. TN22. The results showed that squalene production by the strain Thraustochytrium sp. TN22 was optimum at 2 glucose yeast extract and 0 14 mixture of vitamins B 1 B6 and B12 at 28 oC for 2 days of cultivation. At the optimum conditions the dry biomass squalene content and productivity were approximately g L mg g DCW and mg L respectively. Time-dependence on cell lysis and extraction solvents were selected as the extraction parameters. The obtained results showed that the highest squalene production of mg 100 g biomass was obtained in cell lysate at alkaline medium pH 10 at 60 oC for 6 hours and n- hexan was the best solvent for squalene extraction. Squalene was then purified on silica gel column using n-hexan as the mobile phase and 90 purity of squalene was archived. Our obtained results are promising for the commercial productions of various value -added compounds from species belong to the genus Thrautochytrium in Vietnam. Keywords Thraustochytrium culture isolation squalene production. Citation Hoang Thi Minh Hien Le Thi Thom Nguyen Cam Ha Hoang Thi Lan Anh Dang Diem Hong 2020. Optimization of culture conditions for .