Đang chuẩn bị liên kết để tải về tài liệu:
Báo cáo y học: "ISOLATION OF CHLAMYDIA PNEUMONIAE FROM SERUM SAMPLES OF THE PATIENTS WITH ACUTE CORONARY SYNDROME"

Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: ISOLATION OF CHLAMYDIA PNEUMONIAE FROM SERUM SAMPLES OF THE PATIENTS WITH ACUTE CORONARY SYNDROME. | Int. J. Med. Sci. 2010 7 181 International Journal of Medical Sciences 2010 7 4 181-190 Ivyspring International Publisher. All rights reserved Research Paper ISOLATION OF CHLAMYDIA PNEUMONIAE FROM SERUM SAMPLES OF THE PATIENTS WITH ACUTE CORONARY SYNDROME Ivan M Petyaev 1 Nayilia A Zigangirova 2 Alexey M Petyaev 3 Ulia P Pashko 2 Lubov V Didenko 2 Elena U Morgunova 2 Yuriy K Bashmakov 1 M 1. Cambridge Theranostics Ltd Babraham Research Campus Babraham Cambridge CB2 4AT United Kingdom 2. Gamaleya Institute for Epidemiology and Microbiology RAMS 18 Gamaleya Str. Moscow 123098 Russia 3. Rostov-on-Don Medical University. Nahichevanskii 37 Rostov-on-Don Russia H Corresponding author Dr Yuriy K Bashmakov Cambridge Theranostics Ltd. Babraham Research Campus Cambridge CB2 4AT United Kingdom. Telephone 44-797-1598348 Fax 44-122-3240340 Received 2009.12.16 Accepted 2010.06.07 Published 2010.06.10 Abstract BACKGROUND Limited body of evidence suggests that lipopolysaccharide of C. pneumoniae as well as C. pneumoniae-specific immune complexes can be detected and isolated from human serum. The aim of this study was to investigate the presence of viable elementary bodies of C.pneumoniae in serum samples of patients with acute coronary syndrome and healthy volunteers. MATERIAL AND METHODS Serum specimens from 26 healthy volunteers and 56 patients with acute coronary syndrome were examined subsequently by serological C.pneumoniae-specific IgA and IgG PCR-based and bacteriological methods. Conventional nested and TaqMan PCR were used to detect C.pneumoniae genetic markers ompA and 16S rRNA in DNA from serum specimens extracted with different methods. An alternative protocol which included culturing high-speed serum sediments in HL cells and further C.pneumoniae growth evaluation with immunofluorescence analysis and TaqMan PCR was established. Pellet fraction of PCR-positive serum specimens was also examined by immunoelectron microscopy. RESULTS Best efficiency of final PCR product .