tailieunhanh - Báo cáo y học: " Isolation of suppressor genes that restore retrovirus susceptibility to a virus-resistant cell line"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Isolation of suppressor genes that restore retrovirus susceptibility to a virus-resistant cell line | Retrovirology BioMed Central Research Open Access Isolation of suppressor genes that restore retrovirus susceptibility to a virus-resistant cell line Guangxia Gao1 2 and Stephen P Goff 1 Address Department of Biochemistry and Molecular Biophysics Howard Hughes Medical Institute Columbia University College of Physicians and Surgeons New York NY 10032 USA and 2Institute of Microbiology Chinese Academy of Sciences Beijing 100080 China Email Guangxia Gao - gaogx@ Stephen P Goff - goff@ Corresponding author Published 28 September 2004 Received 24 August 2004 Accepted 28 September 2004 Retrovirology 2004 1 30 doi l742-4690-1-30 This article is available from http content 1 1 30 2004 Gao and Goff licensee BioMed Central Ltd. This is an open-access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Genetic selections in mammalian cell lines have recently been developed for the isolation of mutant cells that are refractory to infection by retroviruses. These selections have been used to recover lines that block early postentry stages of infection either before reverse transcription or before nuclear entry. The mechanisms of action of these blocks remain unknown. Results We have devised a method for the selection of genes from cDNA libraries that suppress the block to virus infection and so restore virus susceptibility. The protocol involves the transformation of pools of resistant cells by cDNA expression libraries followed by the selection for rare virus-sensitive cells using multiple rounds of selection after infection by marked viral vector genomes. The suppressor genes were then recovered from these virus sensitive cells and their ability to restore virus susceptibility was confirmed by .

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