tailieunhanh - Báo cáo y học: "Isolation and characterization of human cells resistant to retrovirus infection"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: "Isolation and characterization of human cells resistant to retrovirus infection. | Retrovirology BioMed Central Research Isolation and characterization of human cells resistant to retrovirus infection Patrycja Lech 1 and Nikunj V Somia 2 Open Access Address 1Molecular Cellular Developmental Biology and Genetics Graduate Program University of Minnesota Minneapolis Minnesota USA and 2Dept. of Genetics Cell Biology and Development and the Institute of Human Genetics University of Minnesota Minneapolis Minnesota USA Email Patrycja Lech - lechx001@ NikunjV Somia - somia001@ Corresponding author Published 3 July 2007 Received 18 December 2006 Retrovirology 2007 4 45 doi 1742-4690-4-45 Accepted 3 July 2007 This article is available from http content 4 1 45 2007 Lech and Somia licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Identification of host cell proteins required for HIV-1 infection will add to our knowledge of the life cycle of HIV-1 and in the development of therapeutics to combat viral infection. We and other investigators have mutagenized rodent cells and isolated mutant cell lines resistant to retrovirus infection. Since there are differences in the efficiency of single round infection with VSVG pseudotyped HIV-1 on cells of different species we conducted a genetic screen to isolate human cells resistant to HIV-1 infection. We chemically mutagenized human HeLa cells and validated our ability to isolate mutants at test diploid loci. We then executed a screen to isolate HeLa cell mutants resistant to infection by an HIV-1 vector coding for a toxic gene product. Results We isolated two mutant cell lines that exhibit up to 10-fold resistance to infection by HIV- 1 vectors. We have verified that the cells are resistant to infection and not .