tailieunhanh - Báo cáo y học: "Isolation and characterization of microparticles in sputum from cystic fibrosis patients"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:Isolation and characterization of microparticles in sputum from cystic fibrosis patients. | Porro et al. Respiratory Research 2010 11 94 http content 11 1 94 RESPIRATORY RESEARCH RESEARCH Open Access Isolation and characterization of microparticles in sputum from cystic fibrosis patients Chiara Porro 1 Silvia Lepore1 Teresa Trotta1 Stefano Castellani1 Luigi Ratclif2 Anna Battaglino2 Sante Di Gioia1 Maria C Martínez3 Massimo Conese21 and Angela B Maffione21 Abstract Background Microparticles MPs are membrane vesicles released during cell activation and apoptosis. MPs have different biological effects depending on the cell from they originate. Cystic fibrosis CF lung disease is characterized by massive neutrophil granulocyte influx in the airways their activation and eventually apoptosis. We investigated on the presence and phenotype of MPs in the sputum a rich non-invasive source of inflammation biomarkers of acute and stable CF adult patients. Methods Spontaneous sputum obtained from 21 CF patients 10 acute and 11 stable and 7 patients with primary ciliary dyskinesia PCD was liquefied with Sputasol. MPs were counted visualized by electron microscopy and identified in the supernatants of treated sputum by cytofluorimetry and immunolabelling for leukocyte CD11a granulocyte CD66b and monocyte-macrophage CD11b antigens. Results Electron microscopy revealed that sputum MPs were in the 100-500 nm range and did not contain bacteria confirming microbiological tests. CF sputa contained higher number of MPs in comparison with PCD sputa. Levels of CD11a -and CD66b - but not CD11 b -MPs were significantly higher in CF than in PCD without differences between acute and stable patients. Conclusions In summary MPs are detectable in sputa obtained from CF patients and are predominantly of granulocyte origin. This novel isolation method for MPs from sputum opens a new opportunity for the study of lung pathology in CF. Background In cystic fibrosis CF the lung disease is characterized by high concentrations of neutrophil chemokines such as IL-8 and