tailieunhanh - Báo cáo khoa học: The structural and biological significance of the EAAEAE insert in the a-domain of human neuronal growth inhibitory factor

Human neuronal growth inhibitory factor (hGIF) is able to inhibit the out-growth of neurons. As compared with the amino acid sequences of metallo-thionein 1⁄2, hGIF contains two insertions: a Thr at position 5 and an acidic hexapeptide EAAEAE(55–60) close to the C-terminus. Moreover, all mammalian growth inhibitory factor sequences contain a conserved CPCP(6–9) motif. | ỊFEBS Journal The structural and biological significance of the EAAEAE insert in the a-domain of human neuronal growth inhibitory factor Bin Cai1 Zhi-Chun Ding1 Qi Zhang2 Feng-Yun Ni1 Hui Wang3 Qi Zheng1 Yang Wang4 Guo-Ming Zhou4 Ke-Qiang Wang4 Hong-Zhe Sun3 Hou-Ming Wu2 and Zhong-Xian Huang1 1 ChemicalBiology Laboratory Department of Chemistry Fudan University Shanghai China 2 State Key Laboratory of Bio-organic and NaturalProducts Shanghai Institute of Organic Chemistry Chinese Academy of Sciences China 3 Department of Chemistry University of Hong Kong China 4 Department of Anatomy Histology and Embryology Medical School Fudan University Shanghai China Keywords cell culture EAAEAE insert metallothionein MT molecular dynamics simulation neuronal growth inhibitory factor GIF Correspondence . Huang Department of Chemistry Fudan University Shanghai 200433 China Fax 86 21 65641740 Tel 86 21 65643973 E-mail zxhuang@ . Wu State Key Laboratory of Bio-organic and NaturalProducts Shanghai Instituteof Organic Chemistry Chinese Academy of Sciences Shanghai 200032 China Fax 86 21 54925239 Tel 86 21 54925342 E-mail hmwu@ . Sun Department of Chemistry the University of Hong Kong Pokfulam Road Hong Kong China Fax 852 2857 1586 Tel 852 96851508 E-mail hsun@ Database The structures of the a-domain in Cd7-hGIF and the solo Cd4-a-domain have been deposited in the Protein Data Bank under the numbers 2FJ4 and 2FJ5 respectively These authors contributed equally to this work Received 16 October 2008 revised 19 March 2009 accepted 24 April 2009 doi Human neuronal growth inhibitory factor hGIF is able to inhibit the outgrowth of neurons. As compared with the amino acid sequences of metallo-thionein 1 2 hGIF contains two insertions a Thr at position 5 and an acidic hexapeptide EAAEAE 55-60 close to the C-terminus. Moreover all mammalian growth inhibitory factor sequences contain a conserved CPCP 6-9 motif.