tailieunhanh - Ebook Farm animal proteomics 2014: Part 2

Part 2 book "Farm animal proteomics 2014" includes content: Using shotgun proteomics to understand the effect of feed restriction on the Ovis aries wool proteome, comparative proteomic analysis of muscle tissue from pre-term and term calves, high frequencies of the αS1-casein zero variant and its relation to coagulation properties in milk from Swedish dairy goats, . and other contents. | Part III Proteomics analysis of food from animal origin Using shotgun proteomics to understand the effect of feed restriction on the Ovis aries wool proteome André Martinho Almeida1 2 Jeffrey E. Plowman3 Duane P. Harland3 Ancy Thomas3 Tanya Kilminster4 Tim Scanlon4 John Milton5 Johan Greeff4 Chris Oldham4 and Stefan Clerens3 1IICT Instituto de Investigação Científica Tropical and CIISA Centro Interdisciplinar de Investigação em Sanidade Animal Lisboa Portugal aalmeida@ 2Instituto de Tecnologia Química e Biológica Universidade Nova de Lisboa and IBET Instituto de Biologia Experimental e Tecnológica Oeiras Portugal 3AgResearch Ltd Lincoln New Zealand 4Department of Agriculture and Food Western Australia Perth WA Australia 5University of Western Australia Crawley WA Australia These authors contributed equally to this publication Introduction Sheep wool is an important animal fibre. It is produced throughout the world and assumes a strategic role in the economies of several nations particularly in the southern hemisphere Argentina South Africa and particularly Australia and New Zealand. Wool fibre is obtained solely from sheep Ovis aries the highest value raw wool coming from pure Merino stock. Numerous factors are known to significantly affect animal fibre production and quality season genetics age altitude and nutritional factors among many others. Season and nutrition are frequently intertwined factors as seasonal weight loss results from poor quality feed and the availability of pastures in the dry season and is a major constraint to sheep production in both tropical and Mediterranean regions. Interestingly and to the best of our knowledge no studies seemed to be available on the effect of seasonal weight loss or feed restriction on either wool quality traits or the wool proteome itself. In this study we examined the effects of experimentally induced weight loss on wool protein profiles in Australian Merino ram lambs using isobaric tags for relative and