tailieunhanh - Nghiên cứu xác định loài nấm da gây bệnh ở động vật tại Việt Nam bằng chỉ thị phân tử sử dụng gen nhân

Bài viết Nghiên cứu xác định loài nấm da gây bệnh ở động vật tại Việt Nam bằng chỉ thị phân tử sử dụng gen nhân được nghiên cứu với mục đích nhằm xác định thành phần loài nấm da bằng kỹ thuật sinh học phân tử sử dụng gen ITS1 và ITS4. | Vietnam Journal of Community Medicine Vol. 64 Special Issue 2023 160-167 INSTITUTE OF COMMUNITY HEALTH STUDY ON IDENTIFICATION OF SKIN FUNGUS SPECIES CAUSING DISEASE IN ANIMALS IN VIETNAM BY MOLECULAR MARKERS USING NUCLEAR GENES Nguyen Duc Tan1 Nguyen Van Thoai1 Huynh Vu Vy1 Tran Huong Quynh2 1 Institute of Veterinary Research and Development of Central Vietnam - 227 road 2 4 Nha Trang Khanh Hoa Vietnam 2 Institute of Vaccines and Biological Medical - 9 Pasteur Nha Trang Khanh Hoa Vietnam Received 29 11 2022 Revised 04 01 2023 Accepted 05 02 2023 ABSTRACT Objectives Identification of skin fungus species causing disease in animals in Vietnam by molecular markers using nuclear genes. Subject and Methods A total of 226 goats 645 rabbits and 473 dogs infected with skin fungus disease in the wild were identified and classified by morphological and molecular biology techniques. Results The results showed that goats and rabbits were infected with 6 species of skin fungus species namely Aspergillus flavus Trichophyton mentagrophytes Microsporum gypseum Candida tropicalis Penicillium aculeatum and Mucor plumbeus. Meanwhile dogs infected with 6 species of the above skin fungus species and 2 species of Penicillium citrinum Aspergillus niger. The fungal samples were positive for PCR reaction the sample sizes were about 490 bp P. aculeatum 550 bp M. plumbeus 650 P. citrinum T. mentagrophytes 680 bp A. niger 700 bp A. flavus and 750 bp M. gypseum . Comparing the nucleotide sequence of the ITS1 gene showed that the fungal species originated and on the subjects were different but they had a very high degree of genetic similarity Fungus species have different origins but are all on the same branch on the family tree. Conclusions Goats and rabbits were infected with 6 species dogs infected with 8 species of skin fungus species. The fungal species originated and on the subjects were different but they had a very high degree of genetic similarity Fungus species have .