tailieunhanh - Utility of a new notation to visualize flow cytometry analysis results: First preliminary comparison with immunohistochemistry to detect CD30 expression on T-cell lymphoma cells

It is important to confirm CD30 expression in T-cell lymphoma cases, but immunohistochemical staining for CD30 is not commonly performed and no comparison has been done between the results of flow cytometry (FCM) and immunohistochemical staining for CD30. | Fujishima et al. BMC Cancer 2021 21 1352 https s12885-021-09098-4 RESEARCH Open Access Utility of a new notation to visualize flow cytometry analysis results first preliminary comparison with immunohistochemistry to detect CD30 expression on T-cell lymphoma cells Fumiyoshi Fujishima1 Noriko Fukuhara2 Hiroki Katsushima3 Yasuhiro Nakamura4 Hideo Harigae2 Hironobu Sasano1 5 and Ryo Ichinohasama6 Abstract Background It is important to confirm CD30 expression in T-cell lymphoma cases but immunohistochemical stain- ing for CD30 is not commonly performed and no comparison has been done between the results of flow cytometry FCM and immunohistochemical staining for CD30. Therefore we devised a notation that we termed proportion of immunoreactivity expression for FCM PRIME-F notation based on the cellular proportion showing different antigen- antibody reactivity. Methods We retrospectively compiled 211 cases of T-cell lymphoma assessed via FCM from major hospitals in Miy- agi Prefecture from January 2012 to January 2019 and compared 52 of these cases with the immunohistochemical immunoreactive IR pattern of CD30 PRIME-I notation . The PRIME-F notation was divided into five levels notations starting with - followed by 3 2 and 1 gt correspond to level-I level-II or level-III notations starting with dim cor- respond to level-IV and those starting with or bright correspond to level-V. Results The 52 cases of PRIME-F notation with included 16 cases of peripheral T-cell lymphoma PTCL NOS 3 of follicular T-cell lymphoma FTL 3 of angioimmunoblastic T-cell lymphoma AITL 6 of extranodal NK T-cell lym- phoma nasal type ENKL 18 of adult T-cell lymphoma ATL and 6 cases of anaplastic large cell lymphoma ALCL . Eight of the 52 cases were immunohistochemically CD30-negative. In the PRIME-F level-I to III group excluding false- positive cases 5 out of 23 cases were Fujishima et al. BMC Cancer 2021 21 1352 Page 2 of 12 showed a minor positive correlation. The PRIME .