tailieunhanh - Successful application of site-directed mutagenesis polymerase chain reaction to mutate TMS 11 of the staphylococcal multidrug efflux protein QacA

This research describes the mutation process of the amino acid residues in TMS 11 of QacA using site-direct PCR. In this research, 15 primers were successfully designed for site-directed mutagenesis PCR. The sitemutagenesis PCR was successfully conducted to create 15 qacA mutants. These mutants will be used in further functional research of QacA. |