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Báo cáo y học: " Transcriptional profiling reveals barcode-like toxicogenomic responses in the zebrafish embryo"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Transcriptional profiling reveals barcode-like toxicogenomic responses in the zebrafish embryo. | Open Access Research Transcriptional profiling reveals barcode-like toxicogenomic responses in the zebrafish embryo Lixin Yang Jules R Kemadjou Christian Zinsmeister Matthias Bauer Jessica Legradi Ferenc Muller Michael Pankratz Jens Jakeln and Uwe Strahle Addresses institute of Toxicology and Genetics Forschungszentrum Karlsruhe Postfach 3640 76021 Karlsruhe Germany. Institute for Applied Computer Science Forschungszentrum Karlsruhe Postfach 3640 76021 Karlsruhe Germany. Institute for Measurement and Control Engineering HTWK Leipzig Postfach 30 11 66 04251 Leipzig Germany. Correspondence Uwe Strahle. Email uwe.straehle@itg.fzk.de Published 25 October 2007 Genome Biology 2007 8 R227 doi l0.ll86 gb-2007-8- l0-r227 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2007 8 10 R227 Received 23 July 2007 Revised 17 September 2007 Accepted 2007 Yang et al licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited Abstract Background Early life stages are generally most sensitive to toxic effects. Our knowledge on the action of manmade chemicals on the developing vertebrate embryo is however rather limited. We addressed the toxicogenomic response of the zebrafish embryo in a systematic manner by asking whether distinct chemicals would induce specific transcriptional profiles. Results We exposed zebrafish embryos to a range of environmental toxicants and measured the changes in gene-expression profiles by hybridizing cDNA to an oligonucleotide microarray. Several hundred genes responded significantly to at least one of the 1 1 toxicants tested. We obtained specific expression profiles for each of the chemicals and could predict the identity of the toxicant from the expression profiles .