tailieunhanh - Báo cáo y học: " Gene expression profiling of Hfe-/- liver and duodenum in mouse strains with differing susceptibilities to iron loading: identification of transcriptional regulatory targets of Hfe and potential hemochromatosis modifiers"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Gene expression profiling of Hfe-/- liver and duodenum in mouse strains with differing susceptibilities to iron loading: identification of transcriptional regulatory targets of Hfe and potential hemochromatosis modifiers. | Open Access Gene expression profiling of Hfe-1- liver and duodenum in mouse strains with differing susceptibilities to iron loading identification of transcriptional regulatory targets of Hfe and potential hemochromatosis modifiers Hélène Coppin Ỷ Valérie Darnaud Léon Kautz Delphine Meynard Marc Aubry Jean Mosser Maria Martinez and Marie-Paule Roth Addresses INSERM U563 Centre de Physiopathologie de Toulouse Purpan Toulouse F-31300 France. tUniversité Toulouse III Paul-Sabatier IFR 30 Toulouse F-31400 France. CNRS UMR6061 Génétique et Développement Rennes F-35000 France. Université de Rennes 1 IFR 140 Rennes F-35000 France. Correspondence Marie-Paule Roth. Email roth@ Published 18 October 2007 Genome Biology 2007 8 R221 doi gb-2007-8- l0-r22l The electronic version of this article is the complete one and can be found online at http 2007 8 l0 R22l Received 8 June 2007 Revised l6 October 2007 Accepted l8 October 2007 2007 Coppin et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Hfe disruption in mouse leads to experimental hemochromatosis by a mechanism that remains elusive. Affymetrix GeneChip Mouse Genome 430 microarrays and bioinformatics tools were used to characterize patterns of gene expression in the liver and the duodenum of wild-type and Hfe-deficient B6 and D2 mice two inbred mouse strains with divergent iron loading severity in response to Hfe disruption to clarify the mechanisms of Hfe action and to identify potential modifier genes. Results We identified l 343 transcripts that were upregulated or downregulated in liver and 370 in duodenum of Hfe-1- mice as compared to wild-type mice of the same genetic background. In liver Hfe disruption upregulated .

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