tailieunhanh - Báo cáo y học: "Transcriptional profiling of the acute pulmonary"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:Transcriptional profiling of the acute pulmonary. | Gungor et al. Respiratory Research 2010 11 24 http content 11 1 24 RESPIRATORY RESEARCH RESEARCH Open Access Transcriptional profiling of the acute pulmonary inflammatory response induced by LPS role of neutrophils 1 2 14 13 1 Nejla Gungor Jeroen LA Pennings Ad M Knaapen Roland K Chiu Marco Peluso Roger WL Godschalk FrederikJ Van Schooten1 Abstract Background Lung cancer often develops in association with chronic pulmonary inflammatory diseases with an influx of neutrophils. More detailed information on inflammatory pathways and the role of neutrophils herein is a prerequisite for understanding the mechanism of inflammation associated cancer. Methods In the present study we used microarrays in order to obtain a global view of the transcriptional responses of the lung to LPS in mice which mimics an acute lung inflammation. To investigate the influence of neutrophils in this process we depleted mice from circulating neutrophils by treatment with anti-PMN antibodies prior to LPS exposure. Results A total of 514 genes was greater than differentially expressed in the LPS induced lung inflammation model. 394 of the 514 were up regulated genes mostly involved in cell cycle and immune inflammation related processes such as cytokine chemokine activity and signalling. Down regulated genes represented nonimmune processes such as development metabolism and transport. Notably the number of genes and pathways that were differentially expressed was reduced when animals were depleted from circulating neutrophils confirming the central role of neutrophils in the inflammatory response. Furthermore there was a significant correlation between the differentially expressed gene list and the promutagenic DNA lesion M1dG suggesting that it is the extent of the immune response which drives genetic instability in the inflamed lung. Several genes that were specifically regulated by the presence of activated neutrophils could be identified and these were .