tailieunhanh - Báo cáo y học: "Transcriptional profiling of bovine intervertebral disc cells: implications for identification of normal and degenerate human intervertebral disc cell phenotypes"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Transcriptional profiling of bovine intervertebral disc cells: implications for identification of normal and degenerate human intervertebral disc cell phenotypes. | Minogue et al. Arthritis Research Therapy 2010 12 R22 http content 12 1 R22 RESEARCH ARTICLE Open Access Transcriptional profiling of bovine intervertebral disc cells implications for identification of normal and degenerate human intervertebral disc cell phenotypes Ben M Minogue 1 Stephen M Richardson1 Leo AH Zeef2 Anthony J Freemont1 and Judith A Hoyland 1 Abstract Introduction Nucleus pulposus NP cells have a phenotype similar to articular cartilage AC cells. However the matrix of the NP is clearly different to that of AC suggesting that specific cell phenotypes exist. The aim of this study was to identify novel genes that could be used to distinguish bovine NP cells from AC and annulus fibrosus AF cells and to further determine their expression in normal and degenerate human intervertebral disc IVD cells. Methods Microarrays were conducted on bovine AC AF and NP cells using Affymetrix Genechip Bovine Genome Arrays. Differential expression levels for a number of genes were confirmed by quantitative real time polymerase chain reaction qRT-PCR on bovine AC AF and NP cells as well as separated bovine NP and notochordal NC cells. Expression of these novel markers were further tested on normal human AC AF and NP cells and degenerate AF and NP cells. Results Microarray comparisons between NP AC AF and NP AC identified 34 NP-specific and 49 IVD-specific genes respectively that were differentially expressed 100 fold. A subset of these were verified by qRT-PCR and shown to be expressed in bovine NC cells. Eleven genes SNAP25 KRT8 KRT18 KRT19 CDH2 IBSP VCAN TNMD BASP1 FOXF1 FBLN1 were also differentially expressed in normal human NP cells although to a lesser degree. Four genes SNAP25 KRT8 KRT18 and CDH2 were significantly decreased in degenerate human NP cells while three genes VCAN TNMD and BASP1 were significantly increased in degenerate human AF cells. The IVD negative marker FBLN1 was significantly increased in both degenerate human NP and AF .