tailieunhanh - Báo cáo y học: " Transcriptional profiling of inductive mesenchyme to identify molecules involved in prostate development and disease"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Transcriptional profiling of inductive mesenchyme to identify molecules involved in prostate development and disease. | Open Access Research Transcriptional profiling of inductive mesenchyme to identify molecules involved in prostate development and disease Griet Vanpoucken Brigid Orrn O Cathal Grace Ray Chan George R Ashley Karin Williams Omar E Franco Simon W Hayward and Axel A Thomson Addresses MRC Human Reproductive Sciences Unit The Queens Medical Research Institute Little France Crescent Edinburgh EH16 4TJ UK. Departments of Urologic Surgery and Cancer Biology Vanderbilt University Medical Center 21st Avenue South Nashville TN 372322765 uSa. n These authors contributed equally to this work. Correspondence Axel AThomson. Email Published 8 October 2007 Genome Biology 2007 8 R2l3 doi gb-2007-8- l0-r2l3 The electronic version of this article is the complete one and can be found online at http 2007 8 l0 R2l3 Received 30 March 2007 Revised 3l May 2007 Accepted 8 October 2007 2007 Vanpoucke et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The mesenchymal compartment plays a key role in organogenesis and cells within the mesenchyme stroma are a source of potent molecules that control epithelia during development and tumorigenesis. We used serial analysis of gene expression SAGE to profile a key subset of prostatic mesenchyme that regulates prostate development and is enriched for growth-regulatory molecules. Results SAGE libraries were constructed from prostatic inductive mesenchyme and from the complete prostatic rudiment including inductive mesenchyme epithelium and smooth muscle . By comparing these two SAGE libraries we generated a list of 219 transcripts that were enriched or specific to inductive mesenchyme and that may act as mesenchymal regulators of .