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Báo cáo y học: "The role of Qa-2, the functional homolog of HLA-G, in a Behcet's disease-like mouse model induced by the herpes virus simplex"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: The role of Qa-2, the functional homolog of HLA-G, in a Behcet's disease-like mouse model induced by the herpes virus simplex. | Lee et al. Journal of Inflammation 2010 7 31 http www.journal-inflammation.eom content 7 1 31 JOURNAL OF INFLAMMATION RESEARCH Open Access The role of Qa-2 the functional homolog of HLA-G in a Behcet s disease-like mouse model induced by the herpes virus simplex Meeyoung Lee21 Bunsoon Choit1 Hyuk Jae Kwon 1 Ju A Shim1 Kyung Sook Park2 Eun-So Lee3 and Seonghyang Sohn 1 4 Abstract Background It has been suggested that the HLA-G molecule is a genetic risk factor for Behcet s disease BD . In this study we evaluated the level of Qa-2 a murine nonclassical class I MHC molecule and possible functional homolog of HLA-G to determine if it was associated with various symptoms of BD-like mice. In addition we investigated siRNA small interfering RNA treatment to determine if it inhibited Qa-2 expression thereby changing the symptoms of mice. Methods RNA interference RNAi and vector transfection were employed to manipulate gene expression in vivo in mice. siRNA small interfering RNA or Qa-2 expression vector was applied to inhibit or up-regulate Qa-2 expression respectively. Results The Qa-2 levels in granulocytes were lower in BD-like mice than in normal controls. The silencing of Qa-2 by intravenous injection of siRNA 500 nmol mouse 4 times at 3-day intervals specifically reduced the Qa-2 levels and worsened the BD-like symptoms. Conclusions Silencing Qa-2 by injecting siRNA into mice resulted in deterioration of symptoms in BD-like mice. Background Since HLA-G human leukocyte antigen-G was first detected by Geraghty et al. 1 it has been reported that HLA-G protein is expressed at the feto-maternal interface during pregnancy 2 and on a subset of thymic epithelial cells 3 and that it is also involved in maintenance of tolerance of the maternal immune system toward the semi-allogeneic fetus. HLA-G is also expressed in other tissues such as intestinal mucosa 4 and PBMC 5 . Numerous studies have evaluated the relevance of HLA-G under pathologic conditions such as transplantation