tailieunhanh - Báo cáo y học: "Dual role of interleukin-17 in pannus growth and osteoclastogenesis in rheumatoid arthritis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Dual role of interleukin-17 in pannus growth and osteoclastogenesis in rheumatoid arthritis. | Ito et al. Arthritis Research Therapy 2011 13 R14 http content 13 1 R14 RESEARCH ARTICLE Open Access Dual role of interleukin-17 in pannus growth and osteoclastogenesis in rheumatoid arthritis 1 1 1 12 1 Hiroshi Ito Hidehiro Yamada Toshiko N Shibata Hirofumi Mitomi So Nomoto Shoichi Ozaki Abstract Introduction In a murine model interleukin IL -17 plays a critical role in the pathogenesis of arthritis. There are controversies however regarding whether IL-17 is a proinflammatory mediator in rheumatoid arthritis RA . We previously established an ex vivo cellular model using synovial tissue ST -derived inflammatory cells which reproduced pannus-like tissue growth and osteoclastic activity in vitro. Using this model we investigated the effects of IL-17 on pannus growth and osteoclastogenesis in RA. Methods Inflammatory cells that infiltrated synovial tissue from patients with RA were collected without enzyme digestion and designated as ST-derived inflammatory cells. ST-derived inflammatory cells were cultured in the presence or absence of IL-17 or indomethacin and the morphologic changes were observed for 4 weeks. Cytokines produced in the culture supernatants were measured by using enzyme-linked immunosorbent assay kits. Osteoclastic activity was assessed by the development of resorption pits in calcium phosphate-coated slides. Results Exogenous addition of IL-17 dramatically enhanced the spontaneous production of IL-6 and prostaglandin E2 PGE2 by the ST-derived inflammatory cells while it had no effect on the production of tumor necrosis factor TNF -a and macrophage colony-stimulating factor M-CSF . Furthermore IL-17 did not affect the spontaneous development of pannus-like tissue growth and osteoclastic activity by the ST-derived inflammatory cells. On the other hand IL-17 enhanced pannus-like tissue growth the production of TNF-a and M-CSF and the development of osteoclastic activity in the presence of indomethacin an inhibitor of endogenous .