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Báo cáo y học: "Enhanced late-outgrowth circulating endothelial progenitor cell levels in rheumatoid arthritis and correlation with disease activity"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Enhanced late-outgrowth circulating endothelial progenitor cell levels in rheumatoid arthritis and correlation with disease activity. | Jodon de Villeroché et al. Arthritis Research Therapy 2010 12 R27 http arthritis-research.eom content 12 1 R27 RESEARCH ARTICLE Open Access Enhanced late-outgrowth circulating endothelial progenitor cell levels in rheumatoid arthritis and correlation with disease activity Vanina Jodon de Villeroché 1 Jerome Avouac1 2 Aurélie Ponceau1 Barbara Ruiz1 André Kahan2 Catherine Boileau1 3 Georges Uzan4 and Yannick Allanore 1 2 Abstract Introduction Angiogenesis and vasculogenesis are critical in rheumatoid arthritis RA as they could be a key issue for chronic synovitis. Contradictory results have been published regarding circulating endothelial progenitor cells EPCs in RA. We herein investigated late outgrowth EPC sub-population using recent recommendations in patients with RA and healthy controls. Methods EPCs defined as Lin- 7AAD- CD34 CD133 VEGFR-2 cells were quantified by flow cytometry in peripheral blood mononuclear cells PBMCs from 59 RA patients mean age 54 15 years disease duration 16 11 years and 36 controls mean age 53 19 years free of cardiovascular events and of cardiovascular risk factors. Concomitantly late outgrowth endothelial cell colonies derived from culture of PBMCs were analyzed by colonyforming units CFUs . Results RA patients displayed higher circulating EPC counts than controls median 112 27 to 588 vs. 60 5 to 275 per million Lin- mononuclear cells P 0.0007 . The number of circulating EPCs positively correlated with disease activity reflected by DAS-28 score r 0.43 P 0.0028 and lower counts were found in RA patients fulfilling remission criteria P 0.0069 . Furthermore late outgrowth CFU number was increased in RA patients compared to controls. In RA there was no association between the number of EPCs and serum markers of inflammation or endothelial injury or synovitis. Conclusions Our data based on a well characterized definition of late outgrowth EPCs demonstrate enhanced levels in RA and relationship with disease activity. This supports the .