tailieunhanh - Báo cáo y học: "The matrix-forming phenotype of cultured human meniscus cells is enhanced after culture with fibroblast growth factor 2 and is further stimulated by hypoxia"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: The matrix-forming phenotype of cultured human meniscus cells is enhanced after culture with fibroblast growth factor 2 and is further stimulated by hypoxia. | Available online http content 8 3 R61 Research article The matrix-forming phenotype of cultured human meniscus cells is enhanced after culture with fibroblast growth factor 2 and is further stimulated by hypoxia Adetola B Adesida Lisa M Grady Wasim S Khan and Timothy E Hardingham UK Centre for Tissue Engineering at The Wellcome Trust Centre for Cell-Matrix Research Faculty of Life Sciences Michael Smith Building The University of Manchester Manchester Oxford Road M13 9PT UK Corresponding author TE Hardingham Received 3 Jan 2006 Revisions requested 1 Feb 2006 Revisions received 15 Feb 2006 Accepted 21 Feb 2006 Published 17 Mar 2006 Arthritis Research Therapy 2006 8 R61 doi ar1 929 This article is online at http content 8 3 R61 2006 Adesida et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Human meniscus cells have a predominantly fibrogenic pattern of gene expression but like chondrocytes they proliferate in monolayer culture and lose the expression of type II collagen. We have investigated the potential of human meniscus cells which were expanded with or without fibroblast growth factor 2 FGF2 to produce matrix in three-dimensional cell aggregate cultures with a chondrogenic medium at low 5 and normal 20 oxygen tension. The presence of FGF2 during the expansion of meniscus cells enhanced the re-expression of type II collagen 200-fold in subsequent three-dimensional cell aggregate cultures. This was increased further 400-fold by culture in 5 oxygen. Cell aggregates of FGF2-expanded meniscus cells accumulated more proteoglycan total glycosaminoglycan over 14 days and deposited a collagen II-rich matrix. The .

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