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báo cáo khoa học: "Analysis of the expression pattern of the BCL11B gene and its relatives in patients with T-cell acute lymphoblastic leukemia"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài:Analysis of the expression pattern of the BCL11B gene and its relatives in patients with T-cell acute lymphoblastic leukemia | Huang et al. Journal of Hematology Oncology 2010 3 44 http www.jhoonline.Org content 3 1 44 JOURNAL OF HEMATOLOGY ONCOLOGY RESEARCH Open Access Analysis of the expression pattern of the BCL11B gene and its relatives in patients with T-cell acute lymphoblastic leukemia 1.2 1 1 I 1 1 1.2 r- f 2 - 2 1.3 Xin Huang Shaohua Chen Qi Shen Lijian Yang Bo Li Liye Zhong Suxia Geng Xin Du Yangqiu Li Abstract Background In a human T-cell acute lymphoblastic leukemia T-ALL cell line Molt-4 siRNA-mediated suppression of BCL11B expression was shown to inhibit proliferation and induce apoptosis functions which may be related to genes involved in apoptosis such as TNFSF10 and BCL2L1 and TGF-b pathways such as SPPland CREBBP . Methods The expression levels of the above mentioned genes and their correlation with the BCL11B gene were analyzed in patients with T-ALL using the TaqMan and SYBR Green I real-time polymerase chain reaction technique. Results Expression levels of BCL11B BCL2L1 and CREBBP mRNA in T-ALL patients were significantly higher than those from healthy controls P 0.05 . In T-ALL patients the BCL11B expression level was negatively correlated with the BCL2L1 expression level rs -0.700 P 0.05 and positively correlated with the SPP1 expression level rs 0.683 P 0.05 . In healthy controls the BCL11B expression level did not correlate with the TNFSF10 BCL2L1 SPP1 or CREBBP expression levels. Conclusions Over-expression of BCL11B might play a role in anti-apoptosis in T-ALL cells through up-regulation of its downstream genes BCL2L1 and CREBBP. Background T-cell acute lymphoblastic leukemia T-ALL accounts for 15 of newly diagnosed ALL cases in children and 20-25 of ALL cases in adults 1 2 . Overall these are aggressive malignancies that do not respond well to chemotherapy and have a poorer prognosis than their B-cell counterparts 3 . The development of targeted therapies including monoclonal antibodies and gene therapy continues. Small interfering RNA siRNA is a promising .