tailieunhanh - báo cáo khoa học: " Isolation, identification and expression analysis of salt-induced genes in Suaeda maritima, a natural halophyte, using PCR-based suppression subtractive hybridization"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Isolation, identification and expression analysis of salt-induced genes in Suaeda maritima, a natural halophyte, using PCR-based suppression subtractive hybridization | BMC Plant Biology BioMed Central Open Access Research article Isolation identification and expression analysis of salt-induced genes in Suaeda maritima a natural halophyte using PCR-based suppression subtractive hybridization Binod B Sahu and Birendra P Shaw Address Environmental Biotechnology Laboratory Institute of Life Sciences Nalco Square Bhubaneswar PIN-751023 India Email Binod B Sahu - binodbiharisahu@ Birendra P Shaw-b_p_shaw@ Corresponding author Published 5 June 2009 Received 12 January 2009 BMC Plant Biology 2009 9 69 doi 1471-2229-9-69 Accepted 5 June 2009 This article is available from http l47l-2229 9 69 2009 Sahu and Shaw licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Despite wealth of information generated on salt tolerance mechanism its basics still remain elusive. Thus there is a need of continued effort to understand the salt tolerance mechanism using suitable biotechnological techniques and test plants species to enable development of salt tolerant cultivars of interest. Therefore the present study was undertaken to generate information on salt stress responsive genes in a natural halophyte Suaeda maritima using PCR-based suppression subtractive hybridization PCR-SSH technique. Results Forward and reverse SSH cDNA libraries were constructed after exposing the young plants to 425 mM NaCl for 24 h. From the forward SSH cDNA library 429 high quality ESTs were obtained. BLASTX search and TIGR assembler programme revealed overexpression of 167 unigenes comprising 89 singletons and 78 contigs with ESTs redundancy of . Among the unigenes were found to be of special interest indicating novel function of these genes with regard to salt