tailieunhanh - Báo cáo y học: " Transcriptome analysis of murine thymocytes reveals age-associated changes in thymic gene expression"

Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: Transcriptome analysis of murine thymocytes reveals age-associated changes in thymic gene expression. | Int. J. Med. Sci. 2009 6 51 International Journal of Medical Sciences 2009 6 1 51-64 Ivyspring International Publisher. All rights reserved Research Paper Transcriptome analysis of murine thymocytes reveals age-associated changes in thymic gene expression Ana Lustig1 Arnell Carter1 Dorothy Bertak1 Divya Enika1 Bolormaa Vandanmagsar1 William Wood2 Kevin G. Becker2 Ashani T. Weeraratna1 and Dennis D. Taub1 M 1. Laboratory of Immunology National Institute on Aging-Intramural Research Program National Institutes of Health Baltimore MD. 21224 uSA 2. The Research Resources Branch National Institute on Aging-Intramural Research Program National Institutes of Health Baltimore MD. 21224 USA H Correspondence to Dennis D. Taub . Laboratory of Immunology National Institute on Aging-Intramural Research Program National Institutes of Health 5600 Nathan Shock Drive Baltimore MD. 21224 Phone 410 558-8159 Fax 410 558-8284 Email taubd@ Received Accepted Published Abstract The decline in adaptive immunity naive T-cell output and a contraction in the peripheral T cell receptor TCR repertoire with age are largely attributable to thymic involution and the loss of critical cytokines and hormones within the thymic microenvironment. To assess the molecular changes associated with this loss of thymic function we used cDNA microarray analyses to examine the transcriptomes of thymocytes from mice of various ages ranging from very young 1 month to very old 24 months . Genes associated with various biological and molecular processes including oxidative phosphorylation T- and B- cell receptor signaling and antigen presentation were observed to significantly change with thymocyte age. These include several immunoglobulin chains chemokine and ribosomal proteins annexin A2 vav 1 and several S100 signaling proteins. The increased expression of immunoglobulin genes in aged thymocytes could be attributed to the thymic B cells which were found to be .