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Báo cáo y học: " Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells"

Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells. | Int. J. Med. Sci. 2011 8 56 International Journal of Medical Sciences 2011 8 1 56-67 Ivyspring International Publisher. All rights reserved. Research Paper Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells Xue-Wen Huang V H Rui-Hua Luo 2 Qi Zhao 3 Zhong-Ze Shen 4 Li-Li Huang 1 Xian-Yuan An1 Lan-Jing Zhao 1 Jie Wang 5 Yu-Zheng Huang5 1. Department of Clinical Laboratory Huadong Sanatorium Wuxi Jiangsu Province 214065 China 2. Department of Gastroscopy Huadong Sanatorium Wuxi Jiangsu Province 214065 China 3. Department of Clinical Laboratory People s Hospital Wuxi Jiangsu Province 214023 China 4. Jiangsu Internation Travel Healthcare Center Yangzhou Branch Yangzhou Jiangsu Province 225009 China 5. Jiangsu Institute of Parasitic Diseases Wuxi Jiangsu Province 214063 China Xue-wen Huang and Rui-hua Luo are co-first authors. H Corresponding author Dr. Xue-wen Huang E-mail dochuang@live.cn Received 2010.11.02 Accepted 2011.01.01 Published 2011.01.08 Abstract To investigate the role of ROS in the helicobacter pylori Hp induced mtDNA mutations AGS cells were treated by extracts of Hp1 1638 or Hp11638M. The ROS levels cytochrome C reductions and intracellular ATP levels were measured. The coding region and the D-Loop region were amplified and sequenced. Results showed the ROS levels cytochrome C reduction and mtDNA mutations were markedly increased and cell viability decreased after treatment with both Hp extracts and 616 mutations were detected in D-Loop region and 3 heteroplasmic point mutations in the Cytb gene. No mutations were found in the coding region. The mutation rates of mtDNA D-Loop region were positively correlated with the ROS levels and negatively to the ATP levels. Key words Helicobacter pylori Reactive Oxygen Species Mitochondrial DNA Mutation. Introduction Helicobacter pylori Hp are Gram-negative mi-croaerophilic bacteria. Hp infection represents a key factor in the etiology of various gastrointestinal .