Đang chuẩn bị liên kết để tải về tài liệu:
Báo cáo y học: "A comparative analysis of antibody repertoire against Staphylococcus aureus antigens in Patients with Deep-Seated versus Superficial staphylococcal Infections"

Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: A comparative analysis of antibody repertoire against Staphylococcus aureus antigens in Patients with Deep-Seated versus Superficial staphylococcal Infections. | Int. J. Med. Sci. 2005 2 129 Research paper International Journal of Medical Sciences ISSN 1449-1907 www.medsci.org 2005 2 4 129-136 2005 Ivyspring International Publisher. All rights reserved A comparative analysis of antibody repertoire against Staphylococcus aureus antigens in Patients with Deep-Seated versus Superficial staphylococcal Infections Ashok Kumar 1 2 Pallab Ray 2 Mamta Kanwar 1 2 Meera Sharma 2 Subhash Varma 3 S. 1. Kresge Eye Institute School of Medicine Wayne State University Detroit MI 48201 USA 2. Departments of Medical Microbiology Post Graduate Institute of Medical Education and Research Chandigarh India 160012 3. Internal Medicine Post Graduate Institute of Medical Education and Research Chandigarh India 160012 Corresponding address Dr. Ashok Kumar Kresge Eye Institute Wayne State University School of Medicine 4717 St. Antoine Detroit MI 48201 USA. Tel 313 577-7922 Fax 313 577-7781 E-mail akuma@med.Wayne.edu Received 2005.07.01 Accepted 2005.08.23 Published 2005.10.01 Immunoblot and an enzyme-linked immunosorbent assays were used to evaluate and compare IgG antibodies against S. aureus whole cell lysate cell wall peptidoglycan and lipoteichoic acid to discriminate between deep-seated and superficial S. aureus infection. Serum samples were examined from patients with deep-seated n 25 and superficial n 25 S. aureus infections and 15 healthy controls. Patients with deep-seated infections exhibited a large number of immuno-reactive bands in their IgG immunoblot profile as compared to those with superficial infections and healthy controls. Anti-staphylococcal IgG antibodies that reacted with two antigens of apparent molecular weight 110 and 98 kDa were specifically present in 96 24 25 of patients with deep-seated infections and were absent in superficial and control sera. Moreover other Gram-positive and Gram-negative bacteria did not share these two unique antigens. The ELISA assays revealed significantly elevated levels of IgG antibodies to .