tailieunhanh - Báo cáo y học: "Comparative context analysis of codon pairs on an ORFeome scale"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Comparative context analysis of codon pairs on an ORFeome scale. | Method Open Access Comparative context analysis of codon pairs on an ORFeome scale Gabriela Moura Miguel Pinheiro Raquel Silva Isabel Miranda Vera Afreixo Gaspar Dias Adelaide Freitas José L Oliveira and Manuel AS Santos Addresses Centre for Cell Biology Department of Biology University of Aveiro 3810-193 Aveiro Portugal. institute of Electronics and Telematics Engineering University of Aveiro 3810-193 Aveiro Portugal. Department of Mathematics University of Aveiro 3810-193 Aveiro Portugal. Correspondence ManuelAS Santos. E-mail msantos@ Published 15 February 2005 Genome Biology 2005 6 R28 The electronic version of this article is the complete one and can be found online at http 2005 6 3 R28 Received 24 September 2004 Revised 25 November 2004 Accepted 17 January 2005 2005 Moura et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Codon context is an important feature of gene primary structure that modulates mRNA decoding accuracy. We have developed an analytical software package and a graphical interface for comparative codon context analysis of all the open reading frames in a genome the ORFeome . Using the complete ORFeome sequences of Saccharomyces cerevisiae Schizosaccharomyces pombe Candida albicans and Escherichia coli we show that this methodology permits large-scale codon context comparisons and provides new insight on the rules that govern the evolution of codonpair context. Background The standard genetic code uses 64 codons for only 22 amino acids including the amino acids selenocysteine and pyrroly-sine whose incorporation into protein requires the reassignment of the UGA and UAG stop codons respectively 1 2 . This degeneracy of the genetic code has important implications .