tailieunhanh - Báo cáo y học: "Lessons learned from the initial sequencing of the pig genome: comparative analysis of an 8 Mb region of pig chromosome 17"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Lessons learned from the initial sequencing of the pig genome: comparative analysis of an 8 Mb region of pig chromosome 17. | Research Open Access Lessons learned from the initial sequencing of the pig genome comparative analysis of an 8 Mb region of pig chromosome 17 Elizabeth A Hart Mario Caccamo Jennifer L Harrow Sean J Humphray James GR Gilbert Steve Trevanion Tim Hubbard Jane Rogers and Max F Rothschild Addresses Wellcome Trust Sanger Institute Wellcome Tust Genome Campus Hinxton Cambridge CB10 1SA UK. Centre for Integrated Animal Genomics Kildee Hall Iowa State University Ames IA 50011 USA. Correspondence Elizabeth A Hart. Email eah@ Published 17 August 2007 Genome Biology 2007 8 RI68 doi gb-2007-8-8-rl 68 The electronic version of this article is the complete one and can be found online at http 2007 8 8 R168 Received 1 March 2007 Revised 6 July 2007 Accepted I7 August 2007 2007 Hart et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background We describe here the sequencing annotation and comparative analysis of an 8 Mb region of pig chromosome I7 which provides a useful test region to assess coverage and quality for the pig genome sequencing project. We report our findings comparing the annotation of draft sequence assembled at different depths of coverage. Results Within this region we annotated 7I loci of which 53 are orthologous to human known coding genes. When compared to the syntenic regions in human and mouse chromosome 2 Mb this region was found to be highly conserved with respect to gene order. The most notable difference between the three species is the presence of a large expansion of zinc finger coding genes and pseudogenes on mouse chromosome 2 between Edn3 and Phactr3 that is absent from pig and human. All of our annotation has been made .