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báo cáo hóa học:" Increased human defensine levels hint at an inflammatory etiology of bisphosphonateassociated osteonecrosis of the jaw: An immunohistological study"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Increased human defensine levels hint at an inflammatory etiology of bisphosphonateassociated osteonecrosis of the jaw: An immunohistological study | Stockmann et al. Journal of Translational Medicine 2011 9 135 http www.translational-medicine.eom content 9 1 135 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Increased human defensine levels hint at an inflammatory etiology of bisphosphonate-associated osteonecrosis of the jaw An immunohistological study 1 1 2 1 1 Philipp Stockmann Falk Wehrhan Stephan Schwarz-Furlan Florian Stelzle Susanne Trabert Friedrich W Neukam1 and Emeka Nkenke1 Abstract Background Human p-defensins hBD are antimicrobial peptides that are an integral part of bone innate immunity. Recently it could be shown that expression of hBD-1 -2 and -3 were upregulated in cases of osteomyelitis of the jaws. In order to gain insight into the possible impairment of hBD metabolism in bisphosphonate-associated osteonecrosis of the jaws BONJ the present exploratory study was designed so as to determine the qualitative and quantitative expression of afore mentioned hBDs in BONJ and infected osteoradionecrosis ORN both of which represent inflammatory bone diseases. Methods Bone samples were collected from patients with BONJ n 20 and ORN n 20 . Non-infected healthy bone samples n 20 were included as controls. Immunohistological staining in an autostainer was carried out by the Strept-ABC -method against hBD-1 -2 -3. Specific positive vs. negative cell reaction of osteocytes labeling index near the border of bony resection was determined and counted for quantitative analysis. Number of vital osteocytes vs. empty osteocytes lacunae was compared between groups. Results hBD-1 -2 and -3 could be detected in BONJ as well as ORN and healthy bone samples. Immunoreactivity against hBD-2 and -3 was significantly higher in BONJ than in ORN and healthy jaw bone samples. Number of empty osteocyte lacunae was significantly higher in ORN compared with BONJ P 0.001 . Conclusion Under the condition of BONJ an increased expression of hBD-1 -2 -3 is detectable similarly to the recently described upregulation of .