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Báo cáo y học: " Replication and transcription of human papillomavirus type 58 genome in Saccharomyces cerevisiae"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Replication and transcription of human papillomavirus type 58 genome in Saccharomyces cerevisiae | Li et al. Virology Journal 2010 7 368 http www.virologyj.eom content 7 1 368 J VIROLOGY JOURNAL RESEARCH Open Access Replication and transcription of human papillomavirus type 58 genome in Saccharomyces cerevisiae 1 2 1 1 w I 71 1 1 P s I- 1 1 Jing Li Xiao Wang Juan Liu Hong Wang Xiao-Li Zhang Wei Tang Yun-Dong Sun Xin Wang Xiu-Ping Yu1 Wei-Ming Zhao1 Abstract Background To establish a convenient system for the study of human papillomavirus HPV we inserted a Saccharomyces cerevisiae selectable marker Ura into HPV58 genome and transformed it into yeast. Results HPV58 genome could replicate extrachromosomally in yeast with transcription of its early and late genes. However with mutation of the viral E2 gene HPV58 genome lost its mitotic stability and the transcription levels of E6 and E7 genes were upregulated. Conclusions E2 protein could participate in viral genome maintenance replication and transcription regulation. This yeast model could be used for the study of certain aspects of HPV life cycle. Background Human papillomaviruses are small circular DNA viruses that infect epithelial cells and normally replicate as nuclear plasmids. The life cycle of papillomavirus is tightly linked to epithelial differentiation 1 . Among the high-risk HPV types associated with cervical cancer human papillomavirus type 58 HPV58 plays a more prominent role in Asian countries. HPV58 has been found in 5.9 of cervical cancer patients in China 2 with an unusually high prevalence in cervical cancer patients in specific areas of China 33.3 in Hong Kong 3 and 16.3 in Shanghai 4 . Despite the availability for biological study of few cell lines containing the DNA of high-risk HPVs such as 16 18 and 31 no cell lines or animal models containing HPV58 have been established. Saccharomyces cerevisiae is a species of budding yeast. The cellular mechanism required for DNA replication in S. cerevisiae is similar to that in human cells 5 . Studies have shown yeast to be a versatile organism for the