tailieunhanh - Báo cáo y học: "Critical role of hnRNP A1 in HTLV-1 replication in human transformed T lymphocytes"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Critical role of hnRNP A1 in HTLV-1 replication in human transformed T lymphocytes | Retrovirology BioMed Central Research Open Access Critical role of hnRNP A1 in HTLV-1 replication in human transformed T lymphocytes Elsa Kress 1 Hicham Hachem Baydoun2 Eranẹoise Bex2 Louis Gazzolo1 and Madeleine Duc Dodon 1 Address 1Virologie Humaine INSERM-U412 Ecole Normale Supérieure de Lyon IFR 128 Biosciences Lyon-Gerland 46 allée d ltalie 69364 Lyon Cedex 07 France and 2Laboratory of Microbiology University of Brussels 1 Avenue E. Gryson 1070 Brussels Belgium Email Elsa Kress - ekress@ Hicham Hachem Baydoun - hmahmoud@ Fran oise Bex - fbex@ Louis Gazzolo - Madeleine Duc Dodon - Corresponding author Published 09 February 2005 Received 01 October 2004 Retrovirology 2005 2 8 doi 1742-4690-2-8 Accepted 09 February 2005 This article is available from http content 2 1 8 2005 Kress et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background In this study we have examined the role of heterogeneous nuclear ribonucleoprotein A1 hnRNP A1 in viral gene expression in T lymphocytes transformed by HTLV-1. Results We have previously observed that hnRNP A1 A1 down-modulates the post transcriptional activity of Rex protein of HTLV-1. Here we tested whether the ectopic expression of a dominant negative mutant NLS-A1-HA defective in shuttling activity or knockdown of the hnRNPAI gene using RNA interference could inhibit Rex-mediated export of viral mRNAs in HTLV- 1 producing C91PL T-cells. We show that the expression of NLS-A1-HA does not modify the export of Rex-dependent viral mRNAs. Conversely inhibiting A1 expression in C91PL cells by RNA interference provoked an increase in the Rex-dependent export of .