tailieunhanh - Báo cáo y học: "Inhibition of HIV-1 replication in primary human monocytes by the IκB-αS32/36A repressor of NF-κB"

Inhibition of HIV-1 replication in primary human monocytes by the IκB-αS32/36A repressor of NF-κB | Retrovirology BioMed Central Research Open Access Inhibition of HIV-I replication in primary human monocytes by the IKB-aS32 36A represSor of NF-kB Camillo Palmieri1 Francesca Trimboli1 Antimina Puca2 Giuseppe Fiume2 Giuseppe Scala1 2 and Ileana Quinto 1 2 Address department of Clinical and Experimental Medicine University of Catanzaro Magna Graecia Via T. Campanella 115 88100 Catanzaro Italy and department of Biochemistry and Medical Biotechnology University of Naples Federico II Via S. Pansini 5 80131 Naples Italy Email Camillo Palmieri - cpalmieri@ Francesca Trimboli - trimboli@ Antimina Puca- puca@ Giuseppe Fiume - fiume@ Giuseppe Scala - scala@ Ileana Quinto - quinto@ Corresponding author Published 21 December 2004 Received 13 December 2004 Accepted 21 December 2004 Retrovirology 2004 1 45 doi 1742-4690-1-45 This article is available from http content 1 1 45 2004 Palmieri et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The identification of the molecular mechanisms of human immunodeficiency virus type 1 HIV-1 transcriptional regulation is required to develop novel inhibitors of viral replication. NF-kB transacting factors strongly enhance the HIV SIV expression in both epithelial and lymphoid cells. Controversial results have been reported on the requirement of NF-kB factors in distinct cell reservoirs such as CD4-positive T lymphocytes and monocytes. We have previously shown that IKB-aS32 36A a proteolysis-resistant inhibitor of NF-kB potently inhibits the growth of HIV-1 and SIVmac239 in cell cultures and in the SIV macaque model of AIDS. To further extend these observations we have generated NL AD8 .